Splash dispersal of Phyllosticta citricarpa conidia from infected citrus fruit

• Published in: Scientific reports Vol. 4; no. 1; p. 6568
• Main Authors: Perryman, S. A. M., Clark, S. J., West, J. S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.10.2014; Nature Publishing Group
• Subjects: 14/63; 631/326/193/2538; 631/326/2522; 631/326/421; Biophysical Phenomena - physiology; Citrus sinensis - microbiology; Conidia; Dispersal; Experiments; Humanities and Social Sciences; multidisciplinary; Phyllosticta citricarpa; Plant Diseases - microbiology; Pycnidiospores; Rain; Rainfall; Saccharomycetales - pathogenicity; Science; Spores, Fungal - physiology; Wind
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep06568

Rain-splash dispersal of Phyllosticta citricarpa (syn. Guignardia citricarpa ) conidia (pycnidiospores) from infected oranges was studied in still air and combined with wind. High power microscopy demonstrated the presence of conidia in splash droplets from diseased oranges, which exuded conidia for over one hour during repeated wetting. The largest (5 mm) incident drops produced the highest splashes (up to 41.0 cm). A linear-by-quadratic surface model predicted highest splashes to be 41.91 cm at a horizontal distance of 25.97 cm from the target orange. Large splash droplets contained most conidia (4–5.5 mm splashes averaged 308 conidia), but were splashed <30 cm horizontal distance. Most (80–90%) splashes were <1 mm diameter but carried only 0–4 conidia per droplet. In multiple splash experiments, splashes combined to reach higher maxima (up to 61.7 cm; linear-by-quadratic surface model prediction, 62.1 cm) than in the single splash experiments. In combination with wind, higher wind speeds carried an increasing proportion of splashes downwind travelling horizontally at least 8 m at the highest wind speed tested (7 m/s), due to a small proportion of droplets (<1 mm) being aerosolised. These experiments suggest that P. citricarpa conidia can be dispersed from infected oranges by splashes of water in rainfall events.