Microbial mediation of complex subterranean mineral structures

• Published in: Scientific reports Vol. 5; no. 1; p. 15525
• Main Authors: Tisato, Nicola, Torriani, Stefano F. F., Monteux, Sylvain, Sauro, Francesco, De Waele, Jo, Tavagna, Maria Luisa, D’Angeli, Ilenia M., Chailloux, Daniel, Renda, Michel, Eglinton, Timothy I., Bontognali, Tomaso R. R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 29.10.2015; Nature Publishing Group
• Subjects: 132/124; 38/23; 704/2151/330; 704/2151/3930; Biofilms; Biofilms - growth & development; Calcium Carbonate - chemistry; Calcium Carbonate - metabolism; Caves; Caves - microbiology; Chemical control; Chemotaxis; Gliding; Gravity; Humanities and Social Sciences; Mineralization; multidisciplinary; Nucleation; Physical properties; Science
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep15525

Helictites—an enigmatic type of mineral structure occurring in some caves—differ from classical speleothems as they develop with orientations that defy gravity. While theories for helictite formation have been forwarded, their genesis remains equivocal. Here, we show that a remarkable suite of helictites occurring in Asperge Cave (France) are formed by biologically-mediated processes, rather than abiotic processes as had hitherto been proposed. Morphological and petro-physical properties are inconsistent with mineral precipitation under purely physico-chemical control. Instead, microanalysis and molecular-biological investigation reveals the presence of a prokaryotic biofilm intimately associated with the mineral structures. We propose that microbially-influenced mineralization proceeds within a gliding biofilm which serves as a nucleation site for CaCO 3 and where chemotaxis influences the trajectory of mineral growth, determining the macroscopic morphology of the speleothems. The influence of biofilms may explain the occurrence of similar speleothems in other caves worldwide and sheds light on novel biomineralization processes.