Sites, pathways, and mechanism of absorption of Cu-EDDS complex in primary roots of maize (Zea Mays L.): anatomical, chemical and histochemical analysis

• Published in: Plant and soil Vol. 343; no. 1/2; pp. 303 - 312
• Main Authors: Niu, Liyuan, Shen, Zhenguo, Wang, Chunchun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.06.2011; Springer Netherlands; Springer Nature B.V
• Subjects: Absorption; Acid soils; Acids; Agronomy. Soil science and plant productions; Analytical chemistry; Anatomy & physiology; Animal, plant and microbial ecology; Biological and medical sciences; Biomedical and Life Sciences; Cell culture; Chelating agents; Complex roots; Copper; Corn; Cortex; Ecology; Endodermis; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Grain; Histology; Hydroponics; Injuries; Ions; Life Sciences; Metal complexes; Metals; Physiology; Plant Physiology; Plant roots; Plant Sciences; Plants; Regular Article; Rhizosphere; Root tips; Root zone; Roots; Soil; Soil Science & Conservation; Soil-plant relationships. Soil fertility; Soil-plant relationships. Soil fertility. Fertilization. Amendments; Xylem; Zea mays; Absorption pathways; Maize; Cu-EDDS complex; Rubeanic acid; Absorption sites; Site; Monocotyledones; Vegetals; Zea mays; Root; C4-Type; Copper complex; Anatomy; Cereal crop; Mechanism; Absorption; Acids; Gramineae; Analysis; Angiospermae; Vegetative apparatus; Copper compound; Spermatophyta; Soil plant relation
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-011-0719-9

To study the mechanism of chelant-metal complexes to be absorbed into plant roots in the presence of different concentration chelating agents, the sites, pathways, and mechanism of absorption of Cu-EDDS complex ([S, S']-ethylene diamine disuccinic acid) in maize (Zea mays L.) primary roots were systematically studied. The results showed that, at low concentrations of the Cu-EDDS complex (<200 μmol L-1) in hydroponic culture, the complex was passively absorbed mainly from the apoplastic spaces where lateral roots penetrate the endodermis and the cortical region into the root xylem, the lateral root zone were the main absorption sites. At higher concentrations (<3,000 μmol L-1), under hydroponic culture and soil culture conditions, the passage cells, which form a physiological barrier controlling ion absorption, were either injured or killed, and the complex could enter the root xylem. Injury to the physiological barrier was a key factor in the complex being absorbed by roots in substantially larger quantities. In addition, the histochemical analysis of rubeanic acid can also be used for other researches involving Cu, and the negative–pressure measuring device provides a new research tool for studying the apoplastic absorption of other metal–chelating complexes, molecules, and ions.