Enhanced production of carotenoids using a Thraustochytrid microalgal strain containing high levels of docosahexaenoic acid-rich oil
Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30–70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation...
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Published in | Bioprocess and biosystems engineering Vol. 41; no. 9; pp. 1355 - 1370 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.09.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Results to date suggest that microalgal Thraustochytrids family strains can be used to produce high-functional omega-3 rich oil (~ 30–70% of dry cell weight) and carotenoid-based antioxidant pigments simultaneously with value-added bioactive potential. In the present study, we describe the isolation and characterization of a new Thraustochytrid
Schizochytrium
sp. from the west coastal area of Korea. This newly isolated Thraustochytrid, identified as
Schizochytrium
sp. through 18S rRNA analysis and named SH104, simultaneously produces high levels of DHA and carotenoid-based antioxidant pigments. An improved
Schizochytrium
mutant, named SHG104, was obtained from the original host strain by γ-irradiation-induced mutagenesis. Under combined temperature-shift cultivation conditions employing white-light LEDs (light-emitting diodes),
Schizochytrium
sp. SHG104 yielded 10.8 g L
−1
of biomass comprising 45.8% total lipids (32.1% DHA) and 4.6 mg L
−1
of astaxanthin. In addition to DHA, the main fatty acids produced by
Schizochytrium
sp. SHG104 were palmitic acid and a trace of other long-chain fatty acids. The carotenoid profile of SH104 and SHG104 was β-carotene, astaxanthin, canthaxanthin, pheonicoxanthin and echinenone, which analyzed by HPLC and LC/APCI–MS. Furthermore, genomic analysis of
Schizochytrium
and
Aurantiochytrium
microalgae confirmed that the presence of carotenogenesis pathway enzymes and genes including geranylgeranyl diphosphate, phytoene synthase, lycopene cyclase, and cytochrome P450 hydroxylase that necessary for the production of antioxidants via a complete biosynthetic KEGG synthesis pathway. This newly isolated
Schizochytrium
microalga potentially have wide application as a source of antioxidants for astaxanthin-containing pigments, commercial omega-3 lipids and feed additives, such as nutritional supplements for aquaculture. |
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ISSN: | 1615-7591 1615-7605 |
DOI: | 10.1007/s00449-018-1963-7 |