Analysis of key genes of jasmonic acid mediated signal pathway for defense against insect damages by comparative transcriptome sequencing

• Published in: Scientific reports Vol. 5; no. 1; p. 16500
• Main Authors: Yang, Fengshan, Zhang, Yuliang, Huang, Qixing, Yin, Guohua, Pennerman, Kayla K., Yu, Jiujiang, Liu, Zhixin, Li, Dafei, Guo, Anping
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.11.2015; Nature Publishing Group
• Subjects: 38/90; 38/91; 631/449/1741/2671; 631/449/2661/2664; Alternative Splicing; Animals; Cluster Analysis; Computational Biology - methods; Cyclopentanes - pharmacology; Data processing; Defensive behavior; Disease Resistance - genetics; Enzymes; Feeding; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Herbivory; High-Throughput Nucleotide Sequencing; Host-Parasite Interactions; Humanities and Social Sciences; Insecta; Jasmonic acid; Leaves; Metabolism; Methyl jasmonate; multidisciplinary; Oxylipins - pharmacology; Plant Leaves - genetics; Plant Leaves - parasitology; Polymorphism, Single Nucleotide; Proteinase; Proteinase inhibitors; Reproducibility of Results; Science; Signal transduction; Signal Transduction - drug effects; Transcription activation; Transcriptome; Volatiles; Zea mays; Zea mays - genetics; Zea mays - parasitology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep16500

Corn defense systems against insect herbivory involve activation of genes that lead to metabolic reconfigurations to produce toxic compounds, proteinase inhibitors, oxidative enzymes and behavior-modifying volatiles. Similar responses occur when the plant is exposed to methyl jasmonate (MeJA). To compare the defense responses between stalk borer feeding and exogenous MeJA on a transcriptional level, we employed deep transcriptome sequencing methods following Ostrinia furnacalis leaf feeding and MeJA leaf treatment. 39,636 genes were found to be differentially expressed with O. furnacalis feeding, MeJA application and O. furnacalis feeding and MeJA application. Following Gene Ontology enrichment analysis of the up- or down- regulated genes, many were implicated in metabolic processes, stimuli-responsive catalytic activity and transfer activity. Fifteen genes that indicated significant changes in the O. furnacalis feeding group: LOX1, ASN1, eIF3, DXS, AOS, TIM, LOX5, BBTI2, BBTI11, BBTI12, BBTI13, Cl-1B, TPS10, DOX and A20/AN1 were found to almost all be involved in jasmonate defense signaling pathways. All of the data demonstrate that the jasmonate defense signal pathway is a major defense signaling pathways of Asian corn borer’s defense against insect herbivory. The transcriptome data are publically available at NCBI SRA: SRS965087.