Changes of sulfur dioxide, nuclear factor-κB, and interleukin-8 levels in pediatric acute lymphoblastic leukemia with bacterial inflammation

• Published in: Chinese medical journal Vol. 127; no. 23; pp. 4110 - 4113
• Main Authors: Wu, Wanshui, Jia, Yongrui, Du, Shuxu, Tang, Hong, Sun, Yangling, Sun, Liming
• Format: Journal Article
• Language: English
• Published: China Department of Pediatrics, Shijitan Hospital, Capital Medical University, Beijing 100038, China%Health Science Center, Peking University, Beijing 100191, China 2014
• Subjects: Adolescent; Bacterial Infections - blood; Bacterial Infections - metabolism; Cell Line; Child; Child, Preschool; Female; Humans; Inflammation - blood; Inflammation - metabolism; Interleukin-8 - blood; Male; NF-kappa B - blood; NF-kappa B - metabolism; Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood; Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism; Sulfur Dioxide - blood; 二氧化硫; 急性; 核因子; 淋巴细胞白血病; 清水; 炎症; 白细胞介素8; 细菌性; nuclear factor-κB; sulfur dioxide; interleukin-8; acute lymphoblastic leukemia; child
• ISSN: 0366-6999; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20133059

Background Bacterial inflammation is a common complication in patients with leukemia,and sulfur dioxide （SO2） is a bioactive molecule in modulating Gram-negative bacilli infection.This study aimed to examine the changes in SO2,nuclear factor-κB （NF-κB）,and interleukin-8 （IL-8） levels in pediatric acute lymphoblastic leukemia （ALL） with Gram-negative bacterial inflammation.Methods Fifty-five ALL children were enrolled in this study,including 30 males and 25 females,aged 3-13 years,and the median age was 7.8 years.All these children who accepted chemotherapy for ALL were divided into the control group （before chemotherapy）,the infection group （after chemotherapy with infection）,and the recovery group （the infection was controlled after 1 week）.The serum level of SO2 was detected using high performance liquid chromatography with fluorescence assay,and NF-κB and IL-8 levels were measured by enzyme-linked immunosorbent assay （ELISA）.Human THP-1 cells were cultured,induced,and differentiated into macrophages,which were divided into five groups and each group was cultured with different stimulators：lipopolysaccharide （LPS） group,LPS＋L-aspartate-β-hydroxamate （HDX） group,LPS＋SO2 group,SO2,and control groups.NF-κB level and IL-8 protein contents by ELISA were examined in each group.Results In comparison with those of the control group,levels of serum SO2,NF-κB,and IL-8 of the infection group were significantly increased （P ＜0.05）,while those of the recovery group were significantly decreased （P ＜0.05）.A positive correlation was found between levels of serum SO2 and intracellular NF-κB/IL-8,and the correlation coefficients were 0.671 and 0.798 （P ＜0.05）,respectively.According to the results found in human THP-1 cells,levels of NF-κB and IL-8 in LPS group were significantly increased compared with those of the control group （P ＜0.05）; when compared with those in LPS group,levels of NF-κB in LPS＋HDX group further increased significantly （P ＜0.05）; however,the NF-κB levels of LPS＋SO2 group decreased significantly （P ＜0.05）.Conclusion SO2 may play an anti-inflammatory role during the process of inflammation by inhibiting the activation and transcription of NF-κB.