DNA MICROSATELLITE ANALYSIS FOR PARENTAGE CONTROL IN AUSTRIAN PIGS

We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large W...

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Bibliographic Details
Published inAnimal biotechnology Vol. 12; no. 2; pp. 141 - 144
Main Authors Nechtelberger, D., Kaltwasser, C., Stur, I., Meyer, J.-N., Brem, G., Mueller, M., Mueller, S.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis Group 01.11.2001
Subjects
Animal Husbandry
Animals
Automation
Female
Male
Microsatellite Repeats
Pedigree
Polymerase Chain Reaction
Polymorphism, Genetic
Probability
Sus scrofa
Swine - genetics
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Summary:We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.
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ISSN:1049-5398
1532-2378
DOI:10.1081/ABIO-100108340