A Dual Transcriptomic Approach Reveals Contrasting Patterns of Differential Gene Expression During Drought in Arbuscular Mycorrhizal Fungus and Carrot

• Published in: Molecular plant-microbe interactions Vol. 36; no. 12; pp. MPMI04230038R - 832
• Main Authors: Keller-Pearson, Michelle, Bortolazzo, Anthony, Willems, Luke, Smith, Brendan, Peterson, Annika, Ané, Jean-Michel, Silva, Erin M
• Format: Journal Article
• Language: English
• Published: United States American Phytopathological Society 01.12.2023; The American Phytopathological Society
• Subjects: Arbuscular mycorrhizas; carrot; Carrots; Down-regulation; drought; Drought resistance; Fungi; Gene expression; Genes; Host plants; Lipids; mycorrhiza; Plant growth; Potassium; Protein transport; RNA-Seq; Spores; stress; Symbiosis; Transcriptomics; Vegetables; stress; RNA-Seq; drought; mycorrhiza; symbiosis; transcriptomics; carrot
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI-04-23-0038-R

While arbuscular mycorrhizal (AM) fungi are known for providing host plants with improved drought tolerance, we know very little about the fungal response to drought in the context of the fungal-plant relationship. In this study, we evaluated the drought responses of the host and symbiont, using the fungus with carrot ( ) as a plant model. Carrots inoculated with spores of DAOM 197198 were grown in a greenhouse. During taproot development, carrots were exposed to a 10-day water restriction. Compared with well-watered conditions, drought caused diminished photosynthetic activity and reduced plant growth in carrot with and without AM fungi. Droughted carrots had lower root colonization. For , 93% of 826 differentially expressed genes (DEGs) were upregulated during drought, including phosphate transporters, several predicted transport proteins of potassium, and the aquaporin . In contrast, 78% of 2,486 DEGs in AM carrot were downregulated during drought, including the symbiosis-specific genes , , and , which are implicated in lipid transfer from the host to the fungus and were upregulated exclusively in AM carrot during well-watered conditions. Overall, this study provides insight into the drought response of an AM fungus in relation to its host; the expression of genes related to symbiosis and nutrient exchange were downregulated in carrot but upregulated in the fungus. This study reveals that carrot and exhibit contrast in their regulation of gene expression during drought, with carrot reducing its apparent investment in symbiosis and the fungus increasing its apparent symbiotic efforts. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.