Precise hit: adeno-associated virus in gene targeting

Vectors based on the adeno-associated virus (AAV) have attracted much attention as potent gene-delivery vehicles, mainly because of the persistence of this non-pathogenic virus in the host cell and its sustainable therapeutic gene expression. However, virus infection can be accompanied by potentiall...

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Bibliographic Details
Published inNature reviews. Microbiology Vol. 3; no. 11; pp. 837 - 847
Main Authors Jessberger, Rolf, Vasileva, Ana
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.11.2005
Subjects
Adeno-associated virus
Adenoviruses
Animals
Dependovirus - genetics
DNA Repair
Eukaryotic Cells
Gene Targeting
Gene therapy
Genetic aspects
Genetic recombination
Genetic Therapy
Genetic Vectors
Genomes
Health aspects
Methods
Recombination, Genetic
Transduction, Genetic
Vectors (Biology)
Viruses
United States
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Summary:Vectors based on the adeno-associated virus (AAV) have attracted much attention as potent gene-delivery vehicles, mainly because of the persistence of this non-pathogenic virus in the host cell and its sustainable therapeutic gene expression. However, virus infection can be accompanied by potentially mutagenic random vector integration into the genome. A novel approach to AAV-mediated gene therapy based on gene targeting through homologous recombination allows efficient, high-fidelity, non-mutagenic gene repair in a host cell.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:1740-1526
1740-1534
DOI:10.1038/nrmicro1266