Effect of LED photobiomodulation on fluorescent light induced changes in cellular ATPases and Cytochrome c oxidase activity in Wistar rat

• Published in: Lasers in medical science Vol. 31; no. 9; pp. 1803 - 1809
• Main Authors: A, Ahamed Basha, C, Mathangi D, R, Shyamala
• Format: Journal Article
• Language: English
• Published: London Springer London 01.12.2016; Springer Nature B.V
• Subjects: ABC transporters; Animals; Calcium-Transporting ATPases - radiation effects; Cells; Cytochromes; Dentistry; Electron Transport Complex IV - radiation effects; Enzyme activity; Enzymes; Exposure; Fluorescence; Fluorescent lighting; Lasers; Light - adverse effects; Light emitting diodes; Low-Level Light Therapy - methods; Male; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Oxidase; Photonics; Physiology; Quantum Optics; Rats; Rats, Wistar; Sodium-Potassium-Exchanging ATPase - radiation effects; Therapy; Time Factors; Cytochrome c oxidase; Na; ATPase; LED; K; Fluorescent light; Ca; Ca2+ ATPase; Na+ K+ ATPase
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-016-2054-0

Background Fluorescent light exposure at night alters cellular enzyme activities resulting in health defects. Studies have demonstrated that light emitting diode photobiomodulation enhances cellular enzyme activities. Objective The objectives of this study are to evaluate the effects of fluorescent light induced changes in cellular enzymes and to assess the protective role of pre exposure to 670 nm LED in rat model. Methods Male Wistar albino rats were divided into 10 groups of 6 animals each based on duration of exposure (1, 15, and 30 days) and exposure regimen (cage control, exposure to fluorescent light [1800 lx], LED preexposure followed by fluorescent light exposure and only LED exposure). Na + –K + ATPase, Ca 2+ ATPase, and cytochrome c oxidase of the brain, heart, kidney, liver, and skeletal muscle were assayed. Results Animals of the fluorescent light exposure group showed a significant reduction in Na + –K + ATPase and Ca 2+ ATPase activities in 1 and 15 days and their increase in animals of 30-day group in most of the regions studied. Cytochrome c oxidase showed increase in their level at all the time points assessed in most of the tissues. LED light preexposure showed a significant enhancement in the degree of increase in the enzyme activities in almost all the tissues and at all the time points assessed. Conclusions This study demonstrates the protective effect of 670 nm LED pre exposure on cellular enzymes against fluorescent light induced change.