Interaction between Medicago truncatula and Pseudomonas fluorescens: Evaluation of Costs and Benefits across an Elevated Atmospheric CO2

• Published in: PloS one Vol. 7; no. 9; p. e45740
• Main Authors: Lepinay, Clémentine, Rigaud, Thierry, Salon, Christophe, Lemanceau, Philippe, Mougel, Christophe
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 21.09.2012
• Subjects: Abiotic factors; Agricultural sciences; Alfalfa; Bacteria; Bacterial physiology; Biodiversity and Ecology; Biology; Carbon dioxide; Carbon dioxide atmospheric concentrations; Carbon dioxide concentration; Carbon dioxide effects; Carbon dioxide in the atmosphere; Compartments; Cost benefit analysis; Costs; Ecology, environment; Environmental changes; Environmental Sciences; Gnotobiotic; Legumes; Life Sciences; Microorganisms; Mutualism; Nutrition; Organic compounds; Parasitism; Plant growth; Plant nutrition; Plant reproduction; Planting density; Plants (botany); Prairies; Pseudomonas fluorescens; Rhizosphere; Seeds; Soil density; Soil microorganisms; Soil study; Studies; Symbiosis; Trophic relationships; terrestrial ecosystem; development; nitrogen; response; plant; dynamic; soil; Pseudomonas fluorescens; carbon-dioxide; rhizosphere
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0045740

Soil microorganisms play a key role in both plants nutrition and health. Their relation with plant varies from mutualism to parasitism, according to the balance of costs and benefits for the two partners of the interaction. These interactions involved the liberation of plant organic compounds via rhizodeposition. Modification of atmospheric CO2 concentration may affect rhizodeposition and as a consequence trophic interactions that bind plants and microorganisms. Positive effect of elevated CO2 on plants are rather well known but consequences for micoorganisms and their interactions with plants are still poorly understood. A gnotobiotic system has been developed to study the interaction between Medicago truncatula Jemalong J5 and the mutualistic bacteria Pseudomonas fluorescens strain C7R12 under two atmospheric CO2 concentrations: ambient (365 ppm) versus enriched (750 ppm). Costs and benefits for each partner have been determined over time by measuring plant development and growth, the C and N contents of the various plant parts and the density of the bacteria in rhizosphere compartments. Following the increase in CO2, there was a beneficial effect of P. fluorescens C7R12 on development, vegetative growth, and C/N content of M. truncatula. Concerning plant reproduction, an early seed production was noticed in presence of the bacterial strain combined with increased atmospheric CO2 conditions. Paradoxically, this transient increase in seed production was correlated with a decrease in bacterial density in the rhizosphere soil, revealing a cost of increased CO2 for the bacterial strain. This shift of costs-benefits ratio disappeared later during the plant growth. In conclusion, the increase in CO2 concentration modifies transiently the cost-benefit balance in favor of the plant. These results may be explained either by a competition between the two partners or a change in bacterial physiology. The ecosystem functioning depends on the stability of many plant-microbe associations that abiotic factors can disrupt.