Integrated Supercritical Fluid Extraction and Pre-Formulation Process of Punica granatum L. Pericarp Polar Compounds

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 24; p. 8110
• Main Authors: Atwi-Ghaddar, Sirine, Destandau, Emilie, Lesellier, Eric
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.12.2023
• Subjects: Acids; Antioxidants; Box–Behnken design (BBD); Carbon dioxide; Chromatography, Supercritical Fluid - methods; cosmetic ingredient; Cosmetics; eco-extraction; Flavonoids; food waste valorization; Fruits; modified supercritical carbon dioxide; Plant Extracts - pharmacology; Pomegranate; Solvents; Variance analysis; eco-extraction; food waste valorization; cosmetic ingredient; modified supercritical carbon dioxide; Box–Behnken design (BBD)
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28248110

Pomegranate ( L.) is a widely used fruit in the dietary supplement industry due to its richness in bioactive compounds. In this study, an experimental design was applied to optimize supercritical fluid extraction (SFE) of polar compounds of interest (ellagic acid and punicalagins), known for antioxidant and skin care properties from pomegranate's pericarp. The effects of temperature, modifier percentage, and water additive percentage added in the modifier were explored through a Box-Behnken design, followed by a study of the extraction kinetics. The results indicated that 40 °C, 20% EtOH:H O 80:20 , with an extraction duration of 60 min allowed for the highest recovery of the above-mentioned molecules (19.59 mg/g). Due to solubilization issues encountered by the extract, a screening of cosmetic solvents was carried out to solubilize SFE pomegranate extracts and a composition of Gly:H O 80:20 was selected. Furthermore, an integrated SFE pre-formulation process of pomegranate pericarp extract (PPE) was elaborated. This allowed for the recovery of the extracts in cosmetic solvent, avoiding a full evaporation. Finally, the stability of the pre-formulated extracts was evaluated and showed high stability for over 3 months at 5 °C.