Development of non-competitive enzyme-linked immunosorbent assays for mummichog Fundulus heteroclitus gonadotropins – Examining seasonal variations in plasma FSH and LH levels in both sexes

Highlights ► Development of specific and sensitive ELISAs for both FSH and LH. ► Seasonal variations in plasma levels of two GtHs in multiple spawning fishes. ► Indication of distinct correlations between plasma FSH levels and gonadal stages. ► Suggestions of the importance of FSH for various reprod...

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Published inGeneral and comparative endocrinology Vol. 178; no. 3; pp. 463 - 469
Main Authors Shimizu, Akio, Ohkubo, Makoto, Hamaguchi, Masami
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.09.2012
Subjects
animal ovaries
Animals
cross reaction
detection limit
ELISA
Endocrinology & Metabolism
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Female
females
fish
Follicle Stimulating Hormone - blood
follicle-stimulating hormone
FSH
Fundulidae - blood
Fundulus heteroclitus
germ cells
Gonadotropins - blood
luteinizing hormone
Luteinizing Hormone - blood
Male
males
Mummichog
Plasma GTH levels
polyclonal antibodies
rearing
reproduction
Reproduction - physiology
Reproductive cycle
seasonal variation
Seasons
spawning
Mummichog
FSH
LH
Reproductive cycle
Plasma GTH levels
ELISA
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Summary:Highlights ► Development of specific and sensitive ELISAs for both FSH and LH. ► Seasonal variations in plasma levels of two GtHs in multiple spawning fishes. ► Indication of distinct correlations between plasma FSH levels and gonadal stages. ► Suggestions of the importance of FSH for various reproductive events.
Bibliography:http://dx.doi.org/10.1016/j.ygcen.2012.07.003
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2012.07.003