Downregulation of TRPM7 suppressed migration and invasion by regulating epithelial–mesenchymal transition in prostate cancer cells
Metastasis is a leading cause of death in patients with prostate cancer (PCa). Transient receptor potential channel 7 (TRPM7) functions as a Mg 2+ /Ca 2+ -permeable channel as well as a protein kinase that regulate various cellular processes including cell adhesion, migration and survival. However,...
Saved in:
Published in | Medical oncology (Northwood, London, England) Vol. 34; no. 7; p. 127 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.07.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Metastasis is a leading cause of death in patients with prostate cancer (PCa). Transient receptor potential channel 7 (TRPM7) functions as a Mg
2+
/Ca
2+
-permeable channel as well as a protein kinase that regulate various cellular processes including cell adhesion, migration and survival. However, the function of
TRPM7
in metastasis of PCa remains largely unknown. Microarray analysis suggested that calcium signaling pathway was significantly altered in metastatic PCa tissues, compared with benign prostatic hyperplasia tissues. Bioinformatics analysis using microarray data and database for annotation, visualization and integrated discovery database revealed altered genes involved in calcium signaling pathway were significantly upregulated in TRPM7 deficiency PCa cells, which was also confirmed by experimental verification. Therefore, we aim to investigate the role of
TRPM7
in human PCa cell migration and invasion as well as the underlying mechanisms. We observed that TRPM7 was upregulated in PCa cells and tissues compared with prostate hyperplasia cells and tissues. Further investigations suggested that
TRPM7
deficiency suppressed migration and invasion of distinct PCa cell lines while overexpression of
TRPM7
increased migration of PCa cells. In addition, knockdown of
TRPM7
in PCa cells reversed the epithelial–mesenchymal transition (EMT) status, accompanied by downregulation of MMPs and upregulation of E-cadherin. Taken together, our study indicated that downregulation of
TRPM7
could inhibit migration and invasion via reversing EMT status in PCa cells. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1357-0560 1559-131X |
DOI: | 10.1007/s12032-017-0987-1 |