Evaluation of blood collection filter papers for HIV-1 DNA PCR

• Published in: Journal of clinical virology Vol. 55; no. 2; pp. 101 - 106
• Main Authors: Masciotra, Silvina, Khamadi, Samoel, Bilé, Ebi, Puren, Adrian, Fonjungo, Peter, Nguyen, Shon, Girma, Mulu, Downing, Robert, Ramos, Artur, Subbarao, Shambavi, Ellenberger, Dennis
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2012; Elsevier
• Subjects: Africa; Allergy and Immunology; Biological and medical sciences; Blood - virology; Blood collection papers; DBS; Desiccation; DNA, Viral - blood; Early diagnosis; Fundamental and applied biological sciences. Psychology; HIV Infections - diagnosis; HIV Infections - virology; HIV-1 - genetics; HIV-1 - isolation & purification; HIV-1 DNA; Human viral diseases; Humans; Humidity; Infant; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; PCR; Polymerase Chain Reaction - methods; Specimen Handling - methods; Temperature; Time Factors; Viral diseases; Virology; Africa; Blood collection papers; HIV-1 DNA; Early diagnosis; DBS; PCR; HIV-1 virus; Retroviridae; Lentivirus; Blood; Virus; Polymerase chain reaction; Human immunodeficiency virus; Diagnosis
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2012.06.010

Abstract Background The collection of dried blood spots (DBS) on Whatman 903 cards has facilitated for years the detection of HIV-1 in infants by DNA PCR as early as 4–6 weeks after birth in resource-limited settings (RLS), but alternate blood collection devices are proving to be necessary. Objectives The qualitative detection of HIV-1 DNA by PCR from DBS prepared on three commercially available blood collection cards was evaluated at the Centers for Disease Control and Prevention (CDC) and in four laboratories in Africa. Study design DBS were prepared on Ahlstrom grade 226, Munktell TFN and Whatman 903, and stored under a variety of conditions. DBS were stored at ambient temperature (RT), 37 °C with high humidity, and −20 °C for varying lengths of time. The presence of HIV-1 DNA was tested using Roche Amplicor HIV-1 DNA (v 1.5) weekly for 4 weeks and at weeks 8 and 12 (RT and 37 °C), at weeks 4, 8, and 18 (−20 °C) of storage. DBS specimens were also tested after international shipment at RT. In addition, after nearly 3 years storage at −20 °C, DBS were also evaluated independently using the COBAS Ampliprep/TaqMan HIV-1 Qual and Abbott RealTime HIV-1 Qualitative tests. Results HIV-1 DNA was detected equally well on the three blood collection cards regardless of storage conditions and PCR assay. Conclusions Ahlstrom 226 and Munktell TFN papers were comparable to Whatman 903 for HIV-1 DNA detection and may be considered as optional blood collection devices in resource-limited countries.