Biocompatibility of KLD-12 Peptide Hydrogel as a Scaffold in Tissue Engineering of Intervertebral Discs in Rabbits

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 2; pp. 173 - 177
• Main Author: 孙建华 郑启新 吴永超 刘郁东 郭晓东 伍卫刚
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.04.2010; Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Orthopedics, the First Affiliated Hospital of Shihezi University, Xinjiang 832008, China%Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Biocompatible Materials; Bone Marrow Cells - cytology; Bone Marrow Cells - physiology; Cell Proliferation - drug effects; Cells, Cultured; Hydrogels - chemistry; Hydrogels - pharmacology; Intervertebral Disc Degeneration - pathology; Intervertebral Disc Degeneration - therapy; Medicine; Medicine & Public Health; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Peptides - chemistry; Rabbits; Tissue Engineering - methods; Tissue Scaffolds; 支架材料; 椎间盘; 水凝胶; 生物相容性; 皮肤刺激试验; 组织工程; 肽合成; self-assembly; peptide; intervertebral disc; biocompatible materials
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-010-0208-z

KLD-12 peptide with a sequence of AcN-KLDLKLDLKLDL-CNH2 was synthesized and its biocompatibility was assessed in animals. Rabbit MSCs were cultured in the hydrogel for 2 weeks. Live cells were counted by using Calcein-AM/P1 fluorescence staining. MTT was employed to assess the viability of MSCs cultured in KLD-12 peptide solution of 0.01%, 0.03%, and 0.05%. Hemolysis test, skin irritation test and implantation test were conducted to evaluate its biocompatibility with host tissues. Our results demonstrated that the MSCs in hydrogel grew well and maintained round shape. Cell survival rate was 92.15% （mean： 92.15%±1.17%） at the 7th day and there was no difference in survival rate between day 7 and day 14. Cell proliferation test showed that the A value of the KLD-12 solutions was not significantly different from that of control groups （complete culture media） （P〉0.05） at the 24th and 48th h. The hemolysis rate of KLD-12 solution was 0.112%. Skin irritation test showed that the skin injected with KLD-12 solution remained normal and the score of skin irritation was 0. The histological examination with HE staining exhibited that the skin layers were clear and there was no infiltration with neutrophilic granulocytes and lymphocytes. It is concluded that KLD-12 peptide hydrogel bad a good biocompatibility with host rabbit and MSCs, and KLD-12 pep- tide hydrogel can provide an appropriate microenvironment for MSCs.