Preparation of a neutral porous monolith and its evaluation in pressurized capillary electrochromatography with neutral and charged solutes
A neutral, nonpolar monolithic capillary column was evaluated as a hydrophobic stationary phase in pressurized CEC system for neutral, acidic and basic solutes. The monolith was prepared by in situ copolymerization of octadecyl methacrylate and ethylene dimethacrylate in a binary porogenic solvent c...
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Published in | Electrophoresis Vol. 31; no. 10; pp. 1674 - 1680 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley-VCH Verlag
01.05.2010
WILEY-VCH Verlag WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | A neutral, nonpolar monolithic capillary column was evaluated as a hydrophobic stationary phase in pressurized CEC system for neutral, acidic and basic solutes. The monolith was prepared by in situ copolymerization of octadecyl methacrylate and ethylene dimethacrylate in a binary porogenic solvent consisting of cyclohexanol/1,4-butanediol. EOF in this hydrophobic monolithic column was poor; even the pH value of the mobile phase was high. Because of the absence of fixed charges, the monolithic capillary column was free of electrostatic interactions with charged solutes. Separations of neutral solutes were based on the hydrophobic mechanism with the pressure as the driving force. The acidic and basic solutes were separated under pressurized CEC mode with the pressure and electrophoretic mobility as the driving force. The separation selectivity of charged solutes were based on their differences in electrophoretic mobility and hydrophobic interaction with the stationary phase, and no obvious peak tailing for basic analytes was observed. Effects of the mobile phase compositions on the retention of acidic compounds were also investigated. Under optimized conditions, high plate counts reaching 82 000 plates/m for neutral compounds, 134 000 plates/m for acid compounds and 150 000 plates/m for basic compounds were readily obtained. |
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Bibliography: | http://dx.doi.org/10.1002/elps.200900669 ArticleID:ELPS200900669 Fujian Province - No. HX2006-99 Natural Science Foundation of Fujian University - No. 2009J0102 Key Science and Technology Project of Fujian Province - No. 2008Y2004; No. 2008Y0020; No. 2006BAK02A21-1-2 istex:5D60B31D73850668D38E010FE2F60F65D72459FA New Century Excellent Talents in University of China - No. NECT-06-0572 National Natural Science Foundation - No. 20907009; No. 40976071 ark:/67375/WNG-JZ6F5HW9-V ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200900669 |