The Effect of Single-Walled Carbon Nanotubes on UDP-Glucuronosyltransferase 1A Activity in Human Liver

• Published in: Biological & pharmaceutical bulletin Vol. 45; no. 4; pp. 446 - 451
• Main Authors: Asai, Yuki, Nadai, Masayuki, Katoh, Miki
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.04.2022; Japan Science and Technology Agency
• Subjects: 17β-Estradiol; Carbon; Drug delivery; Gene transfer; Glucuronides - metabolism; Glucuronosyltransferase; Glucuronosyltransferase - metabolism; hepatic metabolism; Humans; Imipramine; inhibition; Isoforms; kinetics; Liver; Liver - metabolism; Microsomes; Nanotubes; Nanotubes, Carbon; Propofol; Serotonin; UDP-glucuronosyltransferase; Uridine; uridine 5′-diphosphate-glucuronosyltransferase 1A; Uridine Diphosphate; inhibition; uridine 5′-diphosphate-glucuronosyltransferase 1A; kinetics; hepatic metabolism
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b21-00845

Single-walled carbon nanotubes (SWCNTs) are made from rolled single graphene sheets with a diameter in the nanometer range and are potential carriers for drug delivery systems. However, their effects on uridine 5′-diphosphate-glucuronosyltransferase (UGT) 1A activities remain unclear. The present study aimed to investigate the effect of two kinds of SWCNTs (EC1.5-P- and FH-P-SWCNTs) and other nanocarbons on human UGT1A activity due to the proposed application of SWCNTs in drug and gene delivery. β-Estradiol 3-glucuronidation, which is catalyzed mainly by UGT1A1, was inhibited by 99 and 76% in the presence of 0.1 mg/mL EC1.5-P- and FH-P-SWCNTs in human liver microsomes, respectively. The observed decrease of free UGT1A1 protein in the enzyme reaction mixture suggests a higher interaction with SWCNTs, and indicates the inhibition of β-estradiol 3-glucuronidation. Imipramine N-glucuronidation, which is formed mainly by UGT1A4, was also decreased by SWCNTs. Serotonin glucuronidation, which is mainly responsible for UGT1A6, was only influenced by specific nanocarbons in human liver microsomes. The attenuation of free UGT1A6 protein was observed with SWCNTs and carbon black, indicating that UGT1A6 activity was not influenced by the direct interaction of SWCNTs. We also observed a 127% increase by FH-P-SWCNTs for propofol glucuronidation in human liver microsomes, which is catalyzed mainly by UGT1A9. The values of maximum velocity and intrinsic clearance for propofol glucuronidation in the presence of FH-P-SWCNT were 1.8- and 2.0-fold higher than those of the control in human liver microsomes. These results suggest that the effects of SWCNTs on UGT1A are different among isoforms.