In Vitro Secretion Profile of Pro-Inflammatory Cytokines IL-1β, TNF-α, IL-6, and of Human Beta-Defensins (HBD)-1, HBD-2, and HBD-3 from Human Chorioamniotic Membranes After Selective Stimulation with Gardnerella vaginalis
Citation Zaga‐Clavellina V, Martha RV‐M, Flores‐Espinosa P. In vitro secretion profile of pro‐inflammatory cytokines IL‐1β, TNF‐α, IL‐6, and of human beta‐defensins (HBD)‐1, HBD‐2, and HBD‐3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immun...
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Published in | American journal of reproductive immunology (1989) Vol. 67; no. 1; pp. 34 - 43 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.01.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Citation Zaga‐Clavellina V, Martha RV‐M, Flores‐Espinosa P. In vitro secretion profile of pro‐inflammatory cytokines IL‐1β, TNF‐α, IL‐6, and of human beta‐defensins (HBD)‐1, HBD‐2, and HBD‐3 from human chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immunol 2012; 67: 34–43
Problem
Preterm labor associated with infection is a major clinical condition; in this work, we analyze the response of human chorioamniotic membranes stimulated with Gardnerella vaginalis.
Method of study
Using a two‐compartment experimental model, 1 × 106 CFU/mL of G. vaginalis were added to either the amnion or choriodecidua face or to both. Concentrations of IL‐1β, TNF‐α, and IL‐6, as well as human beta defensins (HBD) 1‐3 were quantified by ELISA.
Results
In comparison with control conditions and regardless of the stimulation modality, IL‐1β and IL‐6 increased 4‐fold and 28‐fold, respectively, in the choriodecidual compartment. HBD‐1 increased 2‐fold mainly in the amniotic compartment when the stimulus was applied directly to this region. HBD‐2 and HBD‐3 increased an average of 2‐ and 8‐fold, respectively, in the choriodecidual region.
Conclusions
Stimulation with G. vaginalis induced a tissue‐specific secretion profile of 1L‐1β, IL‐6, and HBD 1‐3 in the chorioamniotic membranes. |
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Bibliography: | ArticleID:AJI1054 istex:CCEB58611BC9C3EFF2C86FCAE45B541F3DF8C5C6 ark:/67375/WNG-R8BZJL9H-6 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1046-7408 1600-0897 |
DOI: | 10.1111/j.1600-0897.2011.01054.x |