Multilayered particle‐packed column: Evaluation and comparison with monolithic and core–shell particle columns for the determination of red azo dyes in Sequential Injection Chromatography

A recently presented new type of “multilayered” organic–inorganic hybrid silica particle packed column YMC‐Triart C18 (50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G,...

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Bibliographic Details
Published inJournal of separation science Vol. 40; no. 6; pp. 1225 - 1233
Main Authors Chocholouš, Petr, Gil, Renato, Acebal, Carolina C., Kubala, Viktor, Šatínský, Dalibor, Solich, Petr
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 01.03.2017
Subjects
Acetonitrile
Azo dyes
Chromatography
columns comparison
Core-shell particles
Dyes
Foods
multilayered particle column
Phases
Separation
sequential injection chromatography
Wavelengths
sequential injection chromatography
columns comparison
multilayered particle column
azo dyes
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Summary:A recently presented new type of “multilayered” organic–inorganic hybrid silica particle packed column YMC‐Triart C18 (50 mm × 4.6 mm, 5 μm) was used for the development of a sequential injection chromatography method for determination of five azo dyes (Sudan I, Sudan II, Sudan III, Sudan orange G, and para red) in selected food seasonings. The use of a novel sorbent brings attractive features, reduced backpressure, and broader chemical stability together with high separation performance, which are discussed and compared with that of three types of columns typically used in medium‐pressure flow chromatography techniques (classic monolithic, narrow monolithic, and core–shell particle columns). The separation was performed in gradient elution mode created by the zone mixing of two mobile phases (acetonitrile/water 90:10, 1.5 mL + acetonitrile/water 100:0, 2.3 mL) at a flow rate of 0.60 mL/min and time of analysis <9.5 min. The spectrophotometric detection wavelengths were set to 400, 480, and 500 nm. The high performance of the developed method with multilayered particle column was well documented and the results indicate a broad capability of sequential injection chromatography.
Bibliography:Conflict of interest: The authors have declared no conflict of interest.
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ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201601224