Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry

A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrati...

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Bibliographic Details
Published inBiological mass spectrometry Vol. 22; no. 4; p. 235
Main Authors Reimer, M L, Mamer, O A, Zavitsanos, A P, Siddiqui, A W, Dadgar, D
Format Journal Article
LanguageEnglish
Published England 01.04.1993
Subjects
Amphetamine - blood
Gas Chromatography-Mass Spectrometry
Humans
Methamphetamine - blood
Quality Control
Regression Analysis
Selegiline - analogs & derivatives
Selegiline - blood
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Summary:A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrations of the three target compounds as their N-heptafluorobutyryl derivatives. Quantitation is performed by stable isotope dilution using d6-amphetamine and d6-methamphetamine as internal standards. Selected ion monitoring of the [M-HF]- ions of both the analytes and internal standards results in minimum quantifiable limits of 0.10 ng ml-1 for both amphetamine and methamphetamine and 0.25 ng ml-1 for desmethyldeprenyl. Excellent linearity (r = 0.998) up to at least 5.00 ng ml-1 is demonstrated.
ISSN:1052-9306
2376-3876
DOI:10.1002/bms.1200220404