Detection by ELISA of IgG-subclass-specific antibodies in primary respiratory syncytial (RS) virus infections

The IgG-subclass specific antibody response was investigated in primary RS-virus infections in infants and small children by using an ELISA with monoclonal antibodies against the four human IgG subclasses. When 78 serum samples obtained from 21 patients during the first 3-4 mo following the onset of...

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Bibliographic Details
Published inJournal of medical virology Vol. 16; no. 4; p. 321
Main Authors Hornsleth, A, Bech-Thomsen, N, Friis, B
Format Journal Article
LanguageEnglish
Published United States 01.08.1985
Subjects
Acute Disease
Antibodies, Monoclonal
Antibodies, Viral - analysis
Bronchitis - immunology
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique
Humans
Immunoenzyme Techniques
Immunoglobulin G - analysis
Infant
Male
Neutralization Tests
Pneumonia, Viral - immunology
Respiratory Syncytial Viruses - immunology
Respiratory Tract Infections - immunology
Respirovirus Infections - immunology
Time Factors
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Summary:The IgG-subclass specific antibody response was investigated in primary RS-virus infections in infants and small children by using an ELISA with monoclonal antibodies against the four human IgG subclasses. When 78 serum samples obtained from 21 patients during the first 3-4 mo following the onset of illness were studied, only IgG-1 and IgG-3 antibodies could be detected. Both of these subclass specific antibodies appeared during the second week following the beginning of illness. Approximately 4 wk after the onset of illness, IgG-1 antibodies reached maximum levels, which persisted during the following months. Approximately 3 wk after the onset of illness, IgG-3 antibodies reached maximum levels, which decreased during the following months. Both IgG-1 and IgG-3 antibodies showed neutralizing capacity.
ISSN:0146-6615
DOI:10.1002/jmv.1890160404