Pulmonary kinetic expression of the endothelin system in a swine model of endotoxic shock

• Published in: Veterinary research communications Vol. 34; no. Suppl 1; pp. 21 - 24
• Main Authors: Zannoni, A, Bernardini, C, Gentilini, F, Giunti, M, Bacci, M. L, Forni, M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.06.2010; Springer Netherlands; Springer Nature B.V
• Subjects: Animals; Aspartic Acid Endopeptidases - genetics; Aspartic Acid Endopeptidases - metabolism; Biomedical and Life Sciences; Dose-Response Relationship, Drug; Endothelin-Converting Enzymes; endothelins; Endothelins - genetics; Endothelins - metabolism; endotoxic shock; endotoxins; Extended Abstract; Female; gene expression; Gene Expression Regulation; Life Sciences; lipopolysaccharides; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - toxicity; Lung - metabolism; Male; messenger RNA; Metalloendopeptidases - genetics; Metalloendopeptidases - metabolism; piglets; poisoning; Receptor, Endothelin A - genetics; Receptor, Endothelin A - metabolism; Receptor, Endothelin B - genetics; Receptor, Endothelin B - metabolism; reverse transcriptase polymerase chain reaction; Shock, Septic - metabolism; Shock, Septic - veterinary; Swine; Swine Diseases - chemically induced; Swine Diseases - metabolism; Veterinary Medicine/Veterinary Science; Zoology; Shock; Endothelin; Swine; Lung; LPS
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-010-9408-z

Endothelin (ET)-1 is a potent vasoconstrictor peptide involved in the derangement of respiratory mechanics during endotoxic shock. We measured the kinetics of pulmonary mRNA expression of the key components of the ET system [i.e., ET-1, ET-converting enzyme (ECE), and ETA and ETB receptors] by quantitative real-time reverse transcriptase-polymerase chain reaction in a swine model of endotoxic shock (0, 1, 2, 3, and 4 h of continuous LPS infusion at 40 μg/kg/hour; sham group, 4 hour saline infusion). A significant increase in mRNA expression levels was observed for ET-1 in LPS-treated piglets; the increase began as early as 1 hour. In contrast, no significant variations were observed for the ECE, ETA, or ETB genes. Small gene expression differences observed with respect to our previous results suggest a possible effect of the anesthesia or surgical protocol on ET system regulation.