Optimization of Saccharomyces boulardii production in solid-state fermentation with response surface methodology

• Published in: Biotechnology, biotechnological equipment Vol. 30; no. 1; pp. 173 - 179
• Main Authors: Hu, Yuanliang, Qin, Huanhuan, Zhan, Zhichun, Dun, Yaohao, Zhou, Ying, Peng, Nan, Ling, Huayun, Liang, Yunxiang, Zhao, Shumiao
• Format: Journal Article
• Language: English
• Published: Sofia Taylor & Francis 02.01.2016; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Ammonium chloride; biotechnology; Box-Behnken design; carbon; Carbon sources; Design factors; experimental design; Fermentation; glucan 1,4-alpha-glucosidase; Glucoamylase; Microbiology; nitrogen; Nitrogen sources; Optimization; Probiotics; response surface method; Response surface methodology; Saccharomyces boulardii; Saccharomyces cerevisiae var. cerevisiae; Solid state; Solid state fermentation; Starch; Statistical methods; Yeast; yeast count; yeasts
• ISSN: 1310-2818; 1314-3530; 1314-3530
• DOI: 10.1080/13102818.2015.1086689

Saccharomyces boulardii preparations are promising probiotics and clinical agents for animals and humans. This work focused on optimizing the nutritional conditions for the production of S. boulardii in solid-state fermentation by using classical and statistical methods. In single-factor experiments, the S. boulardii production was significantly increased by the addition of glucoamylase and the optimal carbon and nitrogen sources were found to be soluble starch and NH 4 Cl, respectively. The effects of the glucoamylase, soluble starch and NH 4 Cl on S. boulardii production were evaluated by a three-level three-factor Box-Behnken design and response surface methodology (RSM). The maximal yeast count (4.50 ×10 9 CFU/g) was obtained under the optimized conditions (198 U/g glucoamylase, 2.37% soluble starch and 0.9% NH 4 Cl), which was in a good agreement with the predicted value of the model. This study has provided useful information on how to improve the accumulation of yeast cells by RSM.