The pattern recognition receptors expressed on neutrophils and the associated cytokine profile from different aged patients with Candida-related denture stomatitis

• Published in: Experimental gerontology Vol. 47; no. 9; pp. 741 - 748
• Main Authors: Gasparoto, Thaís Helena, de Oliveira, Carine Ervolino, Vieira, Narciso Almeida, Porto, Vinicius Carvalho, Gasparoto, Carlos Teodoro, Campanelli, Ana Paula, Lara, Vanessa Soares
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.09.2012
• Subjects: Adult; Age; Aged; Blood; Candida albicans; Candidiasis; Candidiasis, Oral - metabolism; CD69 antigen; Cell activation; Cytokines; Data processing; Dentures; Enzyme-linked immunosorbent assay; Female; Flow Cytometry; Fluorescent Antibody Technique; Geriatrics; Humans; Immune response; Immunofluorescence; Immunosenescence; Interferon-gamma - metabolism; Interleukin 10; Interleukin 12; Interleukin 4; Interleukins - metabolism; Leukocytes (neutrophilic); Male; Middle Aged; Neutrophil; Neutrophils - metabolism; Pattern recognition; Phenotype; Receptors, Pattern Recognition - metabolism; Saliva; Saliva - cytology; Saliva - metabolism; Serum levels; Stomatitis; Stomatitis, Denture - metabolism; TLR2; TLR2 protein; Toll-like receptors; Immunosenescence; Candidiasis; Neutrophil; Cytokines; TLR2
• ISSN: 0531-5565; 1873-6815; 1873-6815
• DOI: 10.1016/j.exger.2012.07.003

This study evaluated the expression of pattern recognition receptors (PRRs) and activation factors associated with salivary and blood neutrophils from different aged patients diagnosed with Candida-related denture stomatitis (DS). Expression of neutrophil PRRs was determined by flow cytometry and immunofluorescence, and the levels of selected cytokines that influence immune activation were determined by ELISA. The salivary (but not the serum derived) neutrophils of individuals with DS were found to have an increased expression of CD69 regardless of the age of the patient compared to patients without DS. However, these salivary neutrophils had a lower expression of CD66b and CD64. Expression of TLR2 was lower on the salivary- and serum-derived neutrophils from elderly individuals compared to the neutrophils of younger subjects, regardless of whether the individual had DS. Salivary interleukin (IL)-4 was elevated in both of the elderly subject groups (with or without DS). Only elderly DS patients were observed to have increased serum IL-4 levels and reduced salivary IL-12 levels. Younger DS patients showed an increase in salivary IL-10 levels, and both the saliva and the serum levels of IFN-γ were increased in all of the younger subjects. Our data demonstrated that changes in both the oral immune cells and the protein components could be associated with DS. Furthermore, changes in the blood-derived factors were more associated with age than DS status. ► IL-4 and IL-10 were increased in the saliva from patients with oral candidiasis. ► IL-12 and IFN-γ were decreased in the saliva from elderly without oral candidiasis. ► TLR2+ neutrophils were found in elderly groups regardless of candidiasis. ► IL-4 was increased in the serum from elderly groups regardless of candidiasis. ► CD69+ salivary neutrophils were increased in both groups with candidiasis.