Caerulein-and xenopsin-related peptides with insulin-releasing activities from skin secretions of the clawed frogs, Xenopus borealis and Xenopus amieti (Pipidae)

• Published in: General and comparative endocrinology Vol. 172; no. 2; pp. 314 - 320
• Main Authors: Zahid, Osama K, Mechkarska, Milena, Ojo, Opeolu O, Abdel-Wahab, Yasser H.A, Flatt, Peter R, Meetani, Mohammed A, Conlon, J. Michael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2011
• Subjects: Animals; Caerulein; Cells, Cultured; Ceruletide - chemistry; Ceruletide - isolation & purification; Ceruletide - metabolism; Ceruletide - pharmacology; Dose-Response Relationship, Drug; Endocrinology & Metabolism; Female; Frog skin secretions; frogs; insulin; Insulin - metabolism; Insulin release; Insulin Secretion; lactate dehydrogenase; Male; Mass spectrometry; Peptide Fragments - isolation & purification; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; peptides; Peptides - chemistry; Peptides - isolation & purification; Peptides - pharmacology; plasma membrane; predators; Rats; Skin - chemistry; Skin - metabolism; tetraploidy; Xenopsin; Xenopus; Xenopus - metabolism; Xenopus Proteins - chemistry; Xenopus Proteins - isolation & purification; Xenopus Proteins - pharmacology; Xenopus; Insulin release; Frog skin secretions; Caerulein; Xenopsin; Mass spectrometry
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2011.03.022

Highlights ► Two sulfatedcaerulein-related peptides (caerulein-B1 and -B2) were isolated from skin secretions of the tetraploid frog Xenopus borealis . ► Caerulein and xenopsin, identical to the X. laevis peptides, and the novel xenopsin-AM2 were isolated from skin secretions of the octoploid frog Xenopus amieti . ► Caerulein-B1, xenopsin, and xenopsin-AM2 produced dose-dependent stimulations of the rate of insulin release from BRIN-BD11 clonal beta cells.