In vitro maturation of baboon oocytes retrieved at the time of cesarean section

• Published in: Fertility and sterility Vol. 71; no. 6; pp. 1153 - 1156
• Main Authors: Brzyski, Robert G, Leland, Michelle M, Eddy, Carlton A
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.06.1999; Elsevier Science
• Subjects: Aging; Animals; Biological and medical sciences; Cells, Cultured; Cesarean Section; Culture Media; epidermal growth factor; Epidermal Growth Factor - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gestational Age; Mammalian reproduction. General aspects; meiosis; Models, Biological; Oocyte physiology; Oocytes - cytology; Oocytes - growth & development; Papio; Pregnancy; Specimen Handling - methods; Vertebrates: reproduction; epidermal growth factor; meiosis; Oocyte physiology; Culture medium; Cell culture; Monkey; Meiosis; Germinal cell; Vertebrata; Randomization; Reproduction; Mammalia; Epidermal growth factor; Primates; Oocyte; Oocyte maturation
• ISSN: 0015-0282; 1556-5653
• DOI: 10.1016/S0015-0282(99)00130-2

Objective: To investigate the feasibility of oocyte retrieval at the time of cesarean delivery and the potential of such oocytes to undergo nuclear maturation in vitro using a baboon model and an established culture system. Design: Randomized, controlled animal study. Setting: Research foundation and university research laboratory. Animal(s): Mature pregnant baboons. Intervention(s): In vitro culture of aspirated oocytes with or without epidermal growth factor (EGF). Main Outcome Measure(s): Oocyte yield, germinal vesicle breakdown, polar body extrusion. Result(s): A total of 246 oocytes were retrieved (mean, 35; range, 14–67). Eighty-seven oocytes (35%) underwent germinal vesicle breakdown and 72 oocytes (29%) extruded a polar body. A χ 2 analysis revealed no significant effect of EGF on outcome parameters. No effect of gestational age or maternal age on oocyte yield or development was observed. Conclusion(s): A sizeable proportion of oocytes obtained from puerperal primates exhibited the capacity to undergo nuclear maturation in vitro.