Paradoxical expression pattern of the epithelial mesenchymal transition-related biomarkers CD44, SLUG, N-cadherin and VSIG1/Glycoprotein A34 in gastrointestinal stromal tumors

AIM To evaluate the immunohistochemical(IHC) expression of five biomarkers, commonly involved in epithelial mesenchymal/mesenchymal epithelial transition(EMT/MET), in gastrointestinal stromal tumors(GISTs). METHODS In 80 consecutive GISTs the IHC examinations were performed using the EMT-related ant...

Full description

Saved in:
Bibliographic Details
Published inWorld journal of gastrointestinal oncology Vol. 9; no. 11; pp. 436 - 443
Main Authors Kövecsi, Attila, Gurzu, Simona, Szentirmay, Zoltan, Kovacs, Zsolt, Bara, Tivadar Jr, Jung, Ioan
Format Journal Article
LanguageEnglish
Published China Baishideng Publishing Group Inc 15.11.2017
Subjects
Retrospective Cohort Study
N-cadherin
V-set and immunoglobulin domain containing gastrointestinal stromal tumors
SLUG
Glycoprotein A34
Online AccessGet full text

Cover

More Information
Summary:AIM To evaluate the immunohistochemical(IHC) expression of five biomarkers, commonly involved in epithelial mesenchymal/mesenchymal epithelial transition(EMT/MET), in gastrointestinal stromal tumors(GISTs). METHODS In 80 consecutive GISTs the IHC examinations were performed using the EMT-related antibodies E-cadherin,N-cadherin, SLUG, V-set and immunoglobulin domain containing 1(VSIG1) and CD44. RESULTS The positivity rate was 88.75% for SLUG, 83.75% for VSIG1, 36.25% for CD44 and 10% for N-cadherin. No correlation was noted between the examined markers and clinicopathological parameters. Nuclear positivity for SLUG and VSIG1 was observed in all cases with distant metastasis. The extra-gastrointestinal stromal tumors(e-GISTs) expressed nuclear positivity for VSIG1 and SLUG, with infrequent positivity for N-cadherin and CD44. The low overall survival was mainly dependent on VSIG1 negativity(P = 0.01) and nuclear positivity for SLUG and/or CD44. CONCLUSION GIST aggressivity may be induced by nuclear upregulation of SLUG and loss or cytoplasm-to-nuclear translocation of VSIG1. SLUG and VSIG1 may act as activated nuclear transcription factors. The CD44, but not N-cadherin, might also have an independent prognostic value in these tumors. The role of the EMT/MET-related transcription factors in the evolution of GISTs, should be revisited with a larger dataset. This is the first study exploring the IHC pattern of VSIG1 in GISTs.
Bibliography:Attila K?vecsi;Simona Gurzu;Zoltan Szentirmay;Zsolt Kovacs;Tivadar Jr Bara;Ioan Jung;Department of Pathology, University of Medicine and Pharmacy;Research Center, University of Medicine and Pharmacy;Department of Pathology, National Institute of Oncology;Department of Biochemistry, University of Medicine and Pharmacy;Department of Surgery, University of Medicine and Pharmacy
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Author contributions: Kovecsi A drafted the article and contributed to interpretation of the immunostains; Gurzu S designed research and contributed to the diagnosis and statistical assessment; Szentirmay Z performed the molecular examinations; Kovacs Z contributed to the molecular examinations; Bara T Jr performed the surgical interventions; Bara T Jr participated at the surgical interventions and the clinical assessment of the cases; Jung I performed the interpretation of the immunohistochemical stains and confer the final agreement for publication; Kövecsi A and Bara T Jr have equal contribution to the paper.
Correspondence to: Simona Gurzu, MD, PhD, Professor, Head of Department of Pathology, University of Medicine and Pharmacy, Gheorghe Marinescu 38 street, Tirgu Mures 540139, Romania. simona.gurzu@umftgm.ro
Telephone: +40-745-673550 Fax: +40-265-210407
ISSN:1948-5204
1948-5204
DOI:10.4251/wjgo.v9.i11.436