Paradoxical expression pattern of the epithelial mesenchymal transition-related biomarkers CD44, SLUG, N-cadherin and VSIG1/Glycoprotein A34 in gastrointestinal stromal tumors
AIM To evaluate the immunohistochemical(IHC) expression of five biomarkers, commonly involved in epithelial mesenchymal/mesenchymal epithelial transition(EMT/MET), in gastrointestinal stromal tumors(GISTs). METHODS In 80 consecutive GISTs the IHC examinations were performed using the EMT-related ant...
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Published in | World journal of gastrointestinal oncology Vol. 9; no. 11; pp. 436 - 443 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
China
Baishideng Publishing Group Inc
15.11.2017
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Subjects | |
Online Access | Get full text |
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Summary: | AIM To evaluate the immunohistochemical(IHC) expression of five biomarkers, commonly involved in epithelial mesenchymal/mesenchymal epithelial transition(EMT/MET), in gastrointestinal stromal tumors(GISTs). METHODS In 80 consecutive GISTs the IHC examinations were performed using the EMT-related antibodies E-cadherin,N-cadherin, SLUG, V-set and immunoglobulin domain containing 1(VSIG1) and CD44. RESULTS The positivity rate was 88.75% for SLUG, 83.75% for VSIG1, 36.25% for CD44 and 10% for N-cadherin. No correlation was noted between the examined markers and clinicopathological parameters. Nuclear positivity for SLUG and VSIG1 was observed in all cases with distant metastasis. The extra-gastrointestinal stromal tumors(e-GISTs) expressed nuclear positivity for VSIG1 and SLUG, with infrequent positivity for N-cadherin and CD44. The low overall survival was mainly dependent on VSIG1 negativity(P = 0.01) and nuclear positivity for SLUG and/or CD44. CONCLUSION GIST aggressivity may be induced by nuclear upregulation of SLUG and loss or cytoplasm-to-nuclear translocation of VSIG1. SLUG and VSIG1 may act as activated nuclear transcription factors. The CD44, but not N-cadherin, might also have an independent prognostic value in these tumors. The role of the EMT/MET-related transcription factors in the evolution of GISTs, should be revisited with a larger dataset. This is the first study exploring the IHC pattern of VSIG1 in GISTs. |
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Bibliography: | Attila K?vecsi;Simona Gurzu;Zoltan Szentirmay;Zsolt Kovacs;Tivadar Jr Bara;Ioan Jung;Department of Pathology, University of Medicine and Pharmacy;Research Center, University of Medicine and Pharmacy;Department of Pathology, National Institute of Oncology;Department of Biochemistry, University of Medicine and Pharmacy;Department of Surgery, University of Medicine and Pharmacy ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: Kovecsi A drafted the article and contributed to interpretation of the immunostains; Gurzu S designed research and contributed to the diagnosis and statistical assessment; Szentirmay Z performed the molecular examinations; Kovacs Z contributed to the molecular examinations; Bara T Jr performed the surgical interventions; Bara T Jr participated at the surgical interventions and the clinical assessment of the cases; Jung I performed the interpretation of the immunohistochemical stains and confer the final agreement for publication; Kövecsi A and Bara T Jr have equal contribution to the paper. Correspondence to: Simona Gurzu, MD, PhD, Professor, Head of Department of Pathology, University of Medicine and Pharmacy, Gheorghe Marinescu 38 street, Tirgu Mures 540139, Romania. simona.gurzu@umftgm.ro Telephone: +40-745-673550 Fax: +40-265-210407 |
ISSN: | 1948-5204 1948-5204 |
DOI: | 10.4251/wjgo.v9.i11.436 |