Aflatoxin production by entomopathogenic isolates of Aspergillus parasiticus and Aspergillus flavus
Fourteen isolates of Aspergillus parasiticus and 2 isolates of Aspergillus flavus isolated from the mealybug Saccharicoccus sacchari were analyzed for production of aflatoxins B 1, B 2, G 1, and G 2 in liquid culture over a 20-day period. Twelve Aspergillus isolates including 11 A.parasiticus and 1...
Saved in:
Published in | Journal of invertebrate pathology Vol. 55; no. 3; pp. 332 - 336 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Inc
01.05.1990
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Fourteen isolates of
Aspergillus parasiticus and 2 isolates of
Aspergillus flavus isolated from the mealybug
Saccharicoccus sacchari were analyzed for production of aflatoxins B
1, B
2, G
1, and G
2 in liquid culture over a 20-day period. Twelve
Aspergillus isolates including 11
A.parasiticus and 1
A.flavus produced aflatoxins which were extracted from both the mycelium and culture filtrate. Aflatoxin production was detected at day 3 and was detected continually up to day 20. Aflatoxin B
1 production was greatest between 7 and 10 days and significantly higher quantities were produced by
A.flavus compared to
A.parasiticus. Aflatoxin production was not a stable trait in 1
A.parasiticus isolate passaged 50 times on agar. In addition to loss of aflatoxin production, an associated loss in sporulation ability was also observed in this passaged isolate, although it did maintain pathogenicity against
S.sacchari. An aflatoxin B
1 concentration of 0.16 μg/mealybug (14.2 μ/g wet wt) was detected within the tissues of infected mealybugs 7 days after inoculation. In conclusion, the ability of
Aspergillus isolates to produce aflatoxins was not essential to the entomopathogenic activity of this fungus against its host
S.sacchari. |
---|---|
Bibliography: | H10 9039098 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0022-2011 1096-0805 |
DOI: | 10.1016/0022-2011(90)90075-H |