Assignment of 20 Microsatellite Markers to the Porcine Linkage Map

• Published in: Genomics (San Diego, Calif.) Vol. 16; no. 2; pp. 431 - 439
• Main Authors: Ellegren, Hans, Johansson, Maria, Chowdhary, Bhanu P., Marklund, Stefan, Ruyter, Dirk, Marklund, Lena, Bräuner-Nielsen, Paul, Edfors-Lilja, Inger, Gustavsson, Ingemar, Juneja, R.Kumar, Andersson, Leif
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.1993; Elsevier
• Subjects: ADN; Animal Breeding and Genetics; Animal Breeding and Genomics; Animals; Base Sequence; Biological and medical sciences; Blood Group Antigens - genetics; CARTE GENETIQUE; CERDO; Chromosome Mapping; CHROMOSOME MAPS; Classical genetics, quantitative genetics, hybrids; Crosses, Genetic; Databases, Factual; DNA; DNA, Satellite - genetics; Fokkerij en Genetica; Fokkerij en Genomica; Fundamental and applied biological sciences. Psychology; Gene Library; Genetic Linkage; GENETIC MAPS; GENETIC MARKERS; GENETIC POLYMORPHISM; GENETICA; GENETICS; Genetics of eukaryotes. Biological and molecular evolution; GENETIQUE; In Situ Hybridization, Fluorescence; Inbreeding; Leerstoelgroep Fokkerij en genetica; LINKAGE GROUPS; LOCI; LOCUS; MAPAS GENETICOS; MARCADORES GENETICOS; MARKER GENES; MARQUEUR GENETIQUE; MOLECULAR MAPPING; Molecular Sequence Data; PIGS; POLIMORFISMO GENETICO; Polymorphism, Restriction Fragment Length; POLYMORPHISME GENETIQUE; PORCIN; SATELLITE DNA; SWINE; Swine - genetics; Vertebrata; Genetic mapping; Vertebrata; Mammalia; Restriction fragment length polymorphism; Genetic marker; Linkage group; Artiodactyla; Ungulata; Pig
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1993.1207

Twenty-one porcine microsatellite markers were developed by screening DNA libraries and by a computer search of databases. The microsatellites were typed in a large three-generation family established by a cross between the European wild pig and a Swedish Yorkshire breed. Linkage analysis benefited from the fact that due to the divergence between the parental populations, the degree of microsatellite polymorphism was significantly higher in the F 1 animals than in either of the parental populations. Parallel typing of a set of 35 restriction fragment length polymorphism, protein, and blood group markers rendered it possible to assign as many as 20 of the microsatellites to the porcine linkage map. Fourteen microsatellites were localized to a chromosome segment, whereas six constituted parts of unassigned linkage groups. Analysis of four microsatellites within genes allowed the assignment of the endoplasmic reticulum Ca 2+ transport ATPase locus to chromosome 14, the assignment of the interferon-γ and the diacylglycerol kinase loci to a new linkage group (XI), and the localization of the tumor necrosis factor β locus close to the major histocompatibility complex (SLA) on chromosome 7 to be confirmed. Fluorescence in situ hybridization mapping of two microsatellite-containing cosmids assigned two linkage groups to chromosomes 9 and 12, respectively. In total, 27 new markers were added to the porcine linkage map, thereby almost doubling the number of markers on the map. Linkage groups are now present on 10 of 18 of the pig autosomes. On chromosome 6, the halothane (HAL) linkage group was extended to about 50 cM, and a similar length was established for the linkage group LPLS0089-ATP2-PLAU on chromosome 14. The results illustrate the usefulness of microsatellite markers in gene mapping and suggest that a saturated linkage map of the porcine genome will be feasible in the near future.