Melatonin Attenuates I/R-Induced Mitochondrial Dysfunction in Skeletal Muscle

• Published in: The Journal of surgical research Vol. 171; no. 1; pp. 108 - 113
• Main Authors: Wang, Wei Z., M.D, Fang, Xin-Hua, M.T, Stephenson, Linda L., M.T, Zhang, Xinlan, B.S, Khiabani, Kayvan T., M.D, Zamboni, William A., M.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.11.2011; Elsevier
• Subjects: Animals; Antioxidants - pharmacology; Benzimidazoles - pharmacokinetics; Biological and medical sciences; Carbocyanines - pharmacokinetics; Cardiology. Vascular system; cytochrome c; Cytochromes c - metabolism; Errors of metabolism; Fluorescent Dyes - pharmacokinetics; General aspects; ischemia/reperfusion; Male; Medical sciences; melatonin; Melatonin - pharmacology; Membrane Potential, Mitochondrial - drug effects; Membrane Potential, Mitochondrial - physiology; Metabolic diseases; Miscellaneous hereditary metabolic disorders; Mitochondria, Muscle - metabolism; mitochondrial membrane potential; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscle, Skeletal - pathology; Rats; Rats, Sprague-Dawley; Reperfusion Injury - drug therapy; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; skeletal muscle; Superoxides - metabolism; Surgery; ischemia/reperfusion; melatonin; cytochrome c; skeletal muscle; mitochondrial membrane potential; Melatonin; Ischemia reperfusion; Electrophysiology; Metabolic diseases; Striated muscle; Enzymopathy; Mitochondrial disorder; Genetic disease; Cytochrome c; Medicine; Mitochondria; Treatment; Surgery; Membrane potential; Pineal hormone
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2010.01.019

Background Our recent studies have shown that ischemia/reperfusion (I/R) produces significant necrosis and apoptosis in the cells of skeletal muscle. Our previous studies also demonstrated that melatonin provides significant protection against superoxide generation, endothelial dysfunction, and cell death in the skeletal muscle after I/R. Mitochondria are essential for cell survival, because of their roles as ATP producers as well as regulators of cell death. However, the efficacy of melatonin on I/R-induced mitochondrial dysfunction in the skeletal muscle in vivo has not been demonstrated in the literature. Materials and Methods Vascular pedicle isolated rat gracilis muscle model was used. After 4 h of ischemia followed by 24 h of reperfusion, gracilis muscle was harvested, and mitochondrial as well as cytosolic fractions were isolated. Mitochondrial dysfunction was determined by the alteration of mitochondrial membrane potential and the release of the proapoptotic protein, cytochrome c . Three groups were designed; sham I/R, I/R-V (I/R with vehicle), and I/R-Mel (I/R with melatonin). Melatonin or vehicle was given intravenously 10 min prior to reperfusion and 10 min after reperfusion. Results We found that the capability of uptake of fluorescent JC-1 dye in skeletal muscle cells was substantially improved in I/R-Mel group compared with I/R-V group. Melatonin significantly inhibited the outflow of cytochrome c from mitochondria to cytoplasm, which was demonstrated in the I/R-V group. Conclusions Melatonin significantly attenuates I/R-induced mitochondrial dysfunction, such as the depolarization of mitochondrial membrane potential and the release of the proapoptotic protein, cytochrome c , from the mitochondria.