An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei

Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with poly...

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Published inRNA (Cambridge) Vol. 9; no. 7; pp. 802 - 808
Main Authors Djikeng, Appolinaire, Shi, Huafang, Tschudi, Christian, Shen, Shuiyuan, Ullu, Elisabetta
Format Journal Article
LanguageEnglish
Published United States Copyright 2003 by RNA Society 01.07.2003
Subjects
Animals
Molecular Weight
Polyribosomes - genetics
Ribonucleoproteins - genetics
RNA, Double-Stranded - genetics
RNA, Protozoan - genetics
RNA, Small Interfering - genetics
RNA, Small Interfering - isolation & purification
Trypanosoma brucei brucei - genetics
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Summary:Genetic interference by double-stranded RNA (dsRNA) or RNA interference (RNAi) triggers the sequence-specific degradation of cellular transcripts. The mediators of mRNA degradation are small interfering RNAs (siRNAs). Here, we report that in Trypanosoma brucei, 10%-20% of siRNAs cosediment with polyribosomes. Preventing the assembly of ribosomes on mRNAs results in a concomitant decrease of siRNAs sedimenting as high-molecular-weight complexes. We further provide evidence that siRNAs are associated with translating ribosomes and that this association is mediated by a 70-kD ribonucleoprotein complex.
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Reprint requests to: Elisabetta Ullu, Department of Internal Medicine, Yale University Medical School, 333 Cedar Street, New Haven, CT 06520-8022, USA; e-mail: elisabetta.ullu@yale.edu; fax: (203) 785-3864.
Article and publication are at http://www.rnajournal.org/cgi/doi/10.1261/rna.5270203.
ISSN:1355-8382
1469-9001
DOI:10.1261/rna.5270203