Classification of Sequences Expressed during the Primordial and Basidiome Stages of the Cultivated Mushroom Agaricus bisporus

• Published in: Fungal genetics and biology Vol. 29; no. 2; pp. 81 - 94
• Main Authors: Ospina-Giraldo, M.D, Collopy, P.D, Romaine, C.P, Royse, D.J
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2000
• Subjects: Agaricus - genetics; Agaricus - growth & development; Agaricus - physiology; Agaricus bisporus; Amino Acid Sequence; cDNA libraries; Expressed Sequence Tags; fungal genomes; gene expression; Gene Expression Regulation, Developmental; Gene Library; genome analysis; Genome, Fungal; Molecular Sequence Data; Monophenol Monooxygenase - genetics; mushroom development; Plasmids; RNA, Messenger - isolation & purification; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Statistics as Topic; fungal genomes; Agaricus bisporus; genome analysis; cDNA libraries; expressed sequence tags; gene expression; mushroom development
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1006/fgbi.2000.1189

Ospina-Giraldo, M. D., Collopy, P. D., Romaine, C. P., and Royse, D. J. 2000. Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus. Two complementary DNA (cDNA) libraries were constructed from tissues isolated from primordia and basidiomes of Agaricus bisporus to characterize genes involved in mushroom development. Using single-pass sequencing of 869 cDNA clones, we found 477 expressed sequence tags (ESTs), including 466 not previously described in the databases for A. bisporus. A BLASTX search revealed that 374 ESTs had similarities with protein sequences available from databases; 193 of these ESTs were categorized according to their putative function. Most ESTs were assigned to one of four roles: metabolism (23%), cell structure (15%), cell growth and division (12%), and protein destination and storage (10%). The remaining ESTs with putative homologues were classified in 10 additional categories. Many ESTs could not be functionally assigned. Based on redundancy levels, at least 4 ESTs were preferentially expressed in each tissue type. Sequence analysis also suggested the presence of paralog tyrosinase genes in the A. bisporus genome.