Induction of Apoptosis of Human Colon Cancer Cells by siRNA Recombinant Expression Vector Targeting Survivin Gene

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 29; no. 1; pp. 45 - 49
• Main Author: 蔡明 王国斌 陶凯雄 蔡昌学
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2009; Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China%Department of Pathogenic Biology,School of Basic Medical Sciences,Ton~i Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
• Subjects: Apoptosis - genetics; Cell Line, Tumor; Colonic Neoplasms - genetics; Colonic Neoplasms - pathology; Genetic Vectors - genetics; Genetic Vectors - metabolism; Humans; Inhibitor of Apoptosis Proteins - genetics; Medicine; Medicine & Public Health; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; RNA Interference; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Small Interfering - genetics; RNA干扰; siRNA; Survivin基因; Transfection; Western印迹; 人结肠癌细胞; 诱导细胞凋亡; 重组表达载体; 靶向Survivin; gene therapy; RNA interference; Survivin
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-009-0109-1

This study constructed siRNA recombinant expression vector targeting survivin gene and observe the apoptosis induction effect of it in human colon cancer cells, siRNA recombinant expression vector targeting survivin gene was constructed and transfected into human colon cancer cells. The effect of siRNA recombinant expression vector was detected by RT-PCR, Western blot, MTT reduction assay and flow cytometry. It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting survivin gene was constructed successfully. Inhibition rate of survivin siRNA at mRNA and protein levels was 36.33% and 44.65% respectively. Growth of cancer cells was inhibited and the apoptosis rate was （17.24±2.13）%. The siRNA recombinant expression vector targeting survivin gene has been constructed successfully. It not only can inhibit the expression of survivin gene, but also can induce apoptosis in human colon cancer cells remarkably.