Direct measurement of homovanillic, vanillylmandelic and 5-hydroxyindoleacetic acids in urine by capillary electrophoresis

Separation conditions in CE, with a neutral coated capillary and reversed polarity, have been optimised to make direct measurement of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid possible in urine samples without pre-treatment. The method developed has been validated, pres...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 871; no. 1; pp. 341 - 350
Main Authors Garcı́a, A, Heinänen, M, Jiménez, L.M, Barbas, C
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 25.02.2000
Elsevier
Subjects
Adult
Biological and medical sciences
Catecholamines
Child
Electrophoresis, Capillary - methods
Homovanillic acid
Homovanillic Acid - urine
Humans
Hydroxyindoleacetic acid
Hydroxyindoleacetic Acid - urine
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Organic acids
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reproducibility of Results
Sensitivity and Specificity
Vanillylmandelic acid
Vanilmandelic Acid - urine
Catecholamines
Vanillylmandelic acid
Homovanillic acid
Hydroxyindoleacetic acid
Organic acids
Human
Urine
Biological fluid
Chemical analysis
Zone electrophoresis
Phase composition
Phenolic acid
Capillary electrophoresis
Metabolite
Patient
Catecholamine
Mobile phase
Vanillyl mandelic acid
Ultraviolet detector
Biogenic amine
Clinical biology
pH
Adult
Child
Quantitative analysis
Indoleacetic acid(5-hydroxy)
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Summary:Separation conditions in CE, with a neutral coated capillary and reversed polarity, have been optimised to make direct measurement of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid possible in urine samples without pre-treatment. The method developed has been validated, presenting adequate parameters for linearity, accuracy and precision. Detection limits range from 0.03 to 2.5 μ M. Finally the method has been applied to urine samples taken from patients, both adults and children, in hospital. Some of them were also measured by immunoassay and HPLC–electrochemical detection and results have been compared.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00994-2