Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma

• Published in: The Journal of immunology (1950) Vol. 140; no. 8; pp. 2625 - 2632
• Main Authors: Rousset, F, Malefijt, RW, Slierendregt, B, Aubry, JP, Bonnefoy, JY, Defrance, T, Banchereau, J, de Vries, JE
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.04.1988; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Antigens, Neoplasm - biosynthesis; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Biological and medical sciences; Burkitt Lymphoma - immunology; Burkitt Lymphoma - pathology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Expression Regulation - drug effects; HLA-D Antigens - biosynthesis; HLA-DQ Antigens - biosynthesis; HLA-DR Antigens - biosynthesis; Humans; Immunobiology; Interferon-gamma - pharmacology; Interleukin-4; Interleukins - antagonists & inhibitors; Interleukins - pharmacology; Lymphokines, interleukins ( function, expression); Receptors, Fc - biosynthesis; Receptors, IgG; Recombinant Proteins - pharmacology; Regulatory factors and their cellular receptors; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - immunology; Human; IgE; Major histocompatibility system; Burkitt lymphoma; Class II histocompatibility antigen; Prostaglandin E2; Immune regulation; Lymphokine; Infection; Antigen; B»-Lymphocyte; Membrane receptor; Regulation(control); Interleukin 4; Cell line; Fc-Fragment; Viral disease; Gamma interferon
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.140.8.2625

The effect of rIL-4 on the expression of low affinity receptor for the Fc part of IgE (Fc epsilon R2/CD23) and class II MHC antigens on Burkitt's lymphoma (BL) cell lines was investigated. Some of the BL lines contained low percentages of CD23 and HLA-DQ-positive cells, but virtually all cells expressed HLA-DR. IL-4 induced CD23 and class II MHC Ag expression on 7 of 9 BL. Optimal CD23 and class II MHC expression was observed after 48-72 h of incubation. Induction of CD23 and class II MHC Ag in the BL cell line BL2 by IL-4 was confirmed at the specific mRNA level. Significant activation of HLA-DQ mRNA was obtained after 6 h of incubation with IL-4 and gradually increased during prolonged incubation. Maximal induction of mRNA transcription occurred after 48 to 72 h. Optimal induction of HLA-DR and CD23 transcription in BL2 was also observed after 48 to 72 h. The induction of CD23 and class II MHC Ag seems to be specific for IL-4, because rIL-1, rIL-2, rIFN-gamma, recombinant granulocyte-macrophage-CSF, and a commercial source of low m.w. B cell growth factor were ineffective. In addition, the expression of class I MHC Ag, the transferrin receptor, CD38, CD25, CD10, CD20, and CD21 were not affected by IL-4. Interestingly, IFN-gamma and PGE2 suppressed the IL-4-induced membrane expression of CD23 and class II MHC Ag in a dose-dependent way. IFN-gamma also blocked IL-4-induced CD23 mRNA transcription in BL2 completely, whereas PGE2 (10(-7) M) was partially inhibitory. The induction of CD23 and class II MHC Ag by IL-4 required intact protein synthesis as shown by its inhibition by cycloheximide. These results indicate that the induction of CD23 and class II MHC Ag by IL-4 is regulated in a coordinated way.