Phylogenetic group distribution and prevalence of virulence genes in Escherichia coli isolates from food samples in South Korea
We analyzed the distribution of phylogenetic groups of foodborne Escherichia coli isolates. We also investigated the prevalence of virulence-associated genes of diarrheagenic E. coli . In total, 162 E. coli isolated from foods (raw meat, fish, and processed foods) were collected in Korea. Approximat...
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Published in | World journal of microbiology & biotechnology Vol. 28; no. 4; pp. 1813 - 1816 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.04.2012
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | We analyzed the distribution of phylogenetic groups of foodborne
Escherichia coli
isolates. We also investigated the prevalence of virulence-associated genes of diarrheagenic
E. coli
. In total, 162
E. coli
isolated from foods (raw meat, fish, and processed foods) were collected in Korea. Approximately 90% of the foodborne isolates belonged to phylogenetic groups A and B1, whereas 1.2% were allocated to group B2, and 9.3% to D. Multiplex polymerase chain reaction (PCR) assays were used to detect the following:
stx
1
and
stx
2
to identify Shiga toxin-producing
E. coli
(STEC),
eae
and
bfpA
to identify enteropathogenic
E. coli
(EPEC),
ipaH
for enteroinvasive
E. coli
, CVD432 for enteroaggregative
E. coli
, and
lt
and
st
for enterotoxigenic
E. coli
(ETEC). The presence of
daaD
in diffusely adherent
E. coli
was examined by singleplex PCR. Of the 162 foodborne
E. coli
isolates, three (1.9%) were confirmed to be pathogenic
E. coli
: STEC, ETEC, and atypical EPEC based on their possession of
stx
1
,
st
, and
eae
, and the pathogenic strains were isolated in beef, rockfish, and pork, respectively. Molecular typing was conducted by multilocus sequence typing to investigate the genetic relationships among the pathogenic strains. All isolates positive for virulence genes had different mulilocus sequence typing profiles representing different sequence types (ST) of ST101, ST1815, and ST1820. These results indicate that some food samples were contaminated with pathogenic
E. coli
. |
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ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-011-0954-5 |