Abscisic Acid Down-Regulates Hydraulic Conductance of Grapevine Leaves in Isohydric Genotypes Only

Plants evolved different strategies to cope with water stress. While isohydric species maintain their midday leaf water potential (ΨM) under soil water deficit by closing their stomata, anisohydric species maintain higher stomatal aperture and exhibit substantial reductions in ΨM. It was hypothesize...

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Published inPlant physiology (Bethesda) Vol. 175; no. 3; pp. 1121 - 1134
Main Authors Coupel-Ledru, Aude, Tyerman, Stephen D., Masclef, Diane, Lebon, Eric, Christophe, Angélique, Edwards, Everard J., Simonneau, Thierry
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.11.2017
Oxford University Press ; American Society of Plant Biologists
Subjects
Abscisic Acid - pharmacology
Down-Regulation - drug effects
ECOPHYSIOLOGY AND SUSTAINABILITY
Genetic Variation
Genotype
Life Sciences
Models, Biological
Plant Exudates - metabolism
Plant Leaves - drug effects
Plant Leaves - physiology
Vegetal Biology
Vitis - drug effects
Vitis - genetics
Vitis - physiology
Water - physiology
ABA
Stomatal conductance
vitis vinifera
Vitis vinifera L
acide abscisique
Isohydric / anisohydric
Transpiration
conductance stomatique
transpiration des feuilles
écophysiologie végétale
conductance hydraulique
abscissins
Leaf hydraulic conductance
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Summary:Plants evolved different strategies to cope with water stress. While isohydric species maintain their midday leaf water potential (ΨM) under soil water deficit by closing their stomata, anisohydric species maintain higher stomatal aperture and exhibit substantial reductions in ΨM. It was hypothesized that isohydry is related to a locally higher sensitivity of stomata to the drought-hormone abscisic acid (ABA). Interestingly, recent lines of evidence in Arabidopsis (Arabidopsis thaliana) suggested that stomatal responsiveness is also controlled by an ABA action on leaf water supply upstream from stomata. Here, we tested the possibility in grapevine (Vitis vinifera) that different genotypes ranging from near isohydric to more anisohydric may have different sensitivities in these ABA responses. Measurements on whole plants in drought conditions were combined with assays on detached leaves fed with ABA. Two different methods consistently showed that leaf hydraulic conductance (Kleaf) was down-regulated by exogenous ABA, with strong variations depending on the genotype. Importantly, variation between isohydry and anisohydry correlated with Kleaf sensitivity to ABA, with Kleaf in the most anisohydric genotypes being unresponsive to the hormone. We propose that the observed response of Kleaf to ABA may be part of the overall ABA regulation of leaf water status.
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A.C.-L., T.S., S.D.T., A.C., and E.L. designed the research; A.C.-L. and D.M. performed the experiments; T.S. and S.D.T. supervised the experiments; E.J.E. supervised ABA analyses; A.C.-L. analyzed the data; A.C.-L. and T.S. wrote the paper with the contribution of S.D.T.
Deceased.
This work was supported by the French programs LACCAVE funded by the “Institut National de la Recherche Agronomique” and ANR-09-GENM-024-002. A.C.-L. received a PhD Grant from the French government. Support for S.D.T. lab is from the Australian Research Council Centre of Excellence in Plant Energy Biology (CE 140100008).
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Thierry Simonneau (thierry.simonneau@inra.fr).
www.plantphysiol.org/cgi/doi/10.1104/pp.17.00698
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.17.00698