Hypertension in Response to AT1-AA: Role of Reactive Oxygen Species in Pregnancy-Induced Hypertension

• Published in: American journal of hypertension Vol. 24; no. 7; pp. 835 - 840
• Main Authors: Parrish, Marc R., Wallace, Kedra, Tam, Kiran B. Tam, Herse, Florian, Weimer, Abram, Wenzel, Katrin, Wallukat, Gerd, Ray, Lillian F., Arany, Marrietta, Cockrell, Kathy, Martin, James N., Dechend, Ralf, LaMarca, Babbette
• Format: Journal Article
• Language: English
• Published: Basingstoke Oxford University Press 01.07.2011; Nature Publishing Group
• Subjects: Animals; Antioxidants - pharmacology; Arterial hypertension. Arterial hypotension; Autoantibodies - physiology; Biological and medical sciences; Blood and lymphatic vessels; Blood Pressure - drug effects; Blood Pressure - physiology; Cardiology. Vascular system; Clinical manifestations. Epidemiology. Investigative techniques. Etiology; Cyclic N-Oxides - pharmacology; Female; Hypertension, Pregnancy-Induced - metabolism; Hypertension, Pregnancy-Induced - physiopathology; Medical sciences; Models, Animal; NADPH Oxidases - pharmacology; Oxidative Stress - drug effects; Oxidative Stress - physiology; Placenta - metabolism; Pregnancy; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Receptor, Angiotensin, Type 1 - immunology; Spin Labels; blood pressure; hypertension; oxidative stress; immune activation; pregnancy; Pregnancy; Hypertension; Oxidative stress; Oxygen; Cardiovascular disease; Arterial pressure; Blood pressure
• ISSN: 0895-7061; 1879-1905; 1941-7225
• DOI: 10.1038/ajh.2011.62

Background Agonistic autoantibodies to the angiotensin II type I receptor (AT1-AA) and reactive oxygen species (ROS) are implicated in the pathophysiology of preeclampsia. The objective of this study was to determine the role of AT1-AA to stimulate placental oxidative stress in vivo and role ROS in mediating hypertension in response to AT1-AA during pregnancy. Methods To achieve these goals, blood pressure (mean arterial pressure (MAP)) and ROS were analyzed in AT1-AA-induced hypertensive pregnant rats in the presence and absence of a superoxide dismutase mimetic, tempol. Rat AT1-AA (1:50) and tempol (30 mg/kg/day) were administered to pregnant rats beginning on day 12 of gestation. On day 19, MAP was analyzed and tissues collected for ROS analysis via lucigenin chemiluminescence. Results MAP increased from 101 ± 2 normal pregnant (NP) rats to 116 ± 2 mm Hg in chronic AT1-AA infused rats (P = 0.002). Placental basal and NADPH oxidase stimulated ROS was 29 ± 6 and 92 ± 10 relative light units (RLUs) in NP rats. These levels increased to 159 ± 29 (P < 0.0001) and 287 ± 60 RLUs (P < 0.006) in AT1-AA infused rats. MAP in AT1-AA + tempol rats was 109 ± 2 mm Hg, no difference than tempol-treated controls (109 ± 3 mm Hg). Administration of tempol decreased basal and NADPH-stimulated placental ROS in AT1-AAtreated rats (121 ± 13; 262 ± 21 RLUs). Basal and NADPH-stimulated ROS in tempol-treated controls were 69 ± 24; 141 ± 33 RLUs. Conclusion This study indicates that AT1-AA’s contribute to placental oxidative stress; one mechanism whereby the AT1-AA mediates hypertension during pregnancy. American Journal of Hypertension advance online publication 7 April 2011; doi:10.1038/ajh.2011.62