A role for peroxidasin PXN-1 in aspects of C. elegans development

• Published in: Molecules and cells Vol. 38; no. 1; pp. 51 - 57
• Main Authors: Lee, Juyeon, Bandyopadhyay, Jaya, Lee, Jin Il, Cho, Injeong, Park, Daeho, Cho, Jeong Hoon
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 01.01.2015; 한국분자세포생물학회
• Subjects: Animals; Animals, Genetically Modified; Caenorhabditis elegans - genetics; Caenorhabditis elegans - growth & development; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Extracellular Matrix - metabolism; Extracellular Matrix Proteins - genetics; Extracellular Matrix Proteins - metabolism; Genes, Lethal; Hot Temperature; Mutation; Neurons - metabolism; Peroxidase - genetics; Peroxidase - metabolism; Peroxidasin; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; 생물학; neuronal development; pxn-1; heat stress; C. elegans; peroxidasins
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2015.2202

The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.