Sequence Characterization of Ndelle Virus Genome Segments 1, 5, 7, 8, and 10: Evidence for Reassignment to the Genus Orthoreovirus, Family Reoviridae

• Published in: Biochemical and biophysical research communications Vol. 287; no. 2; pp. 583 - 588
• Main Authors: Attoui, Houssam, Biagini, Philippe, Stirling, Julie, Mertens, Peter P.C., Cantaloube, Jean-Francois, Meyer, Adam, de Micco, Philippe, de Lamballerie, Xavier
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.09.2001
• Subjects: double-stranded RNA virus; Genome, Viral; Mammalian orthoreovirus; Ndelle virus; NDEV; Orbivirus; orthoreovirus; Orthoreovirus - classification; Orthoreovirus - genetics; Phylogeny; Reoviridae; RNA, Double-Stranded - analysis; RNA, Viral - analysis; taxonomy; double-stranded RNA virus; NDEV; taxonomy; Ndelle virus; orthoreovirus
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.5612

The full-length nucleotide sequences of genome segments 1, 5, 7, 8 and 10 from Ndelle virus (NDEV) have been characterized. Comparison of the deduced protein amino acid sequences with those of other member viruses of the family Reoviridae demonstrates that NDEV was originally assigned incorrectly to the genus Orbivirus (aa identity values of <20%). In contrast, high levels of amino acid identity were found with members of the species Mammalian orthoreovirus (MRV); for example, amino acid identity in γ3(Pol) is between 91 and 97%. These findings, together with previous antigenic analyses, provide evidence that NDEV should be reclassified as a new serotype (designated MRV-4) within the Mammalian orthoreovirus species.