Characterization and antioxidant activity of avenanthramides from selected oat lines developed by mutagenesis technique

• Published in: Food chemistry Vol. 343; p. 128408
• Main Authors: Hernandez-Hernandez, Oswaldo, Pereira-Caro, Gema, Borges, Gina, Crozier, Alan, Olsson, Olof
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2021
• Subjects: Annan teknik; Antioxidant; Antioxidants - analysis; Antioxidants - chemistry; Avena - chemistry; Avena - genetics; Avena sativa; Avenanthramides; Chromatography, High Pressure Liquid - methods; Engineering and Technology; Flour; Food Engineering; Livsmedelsteknik; Mass Spectrometry; Mutagenesis; Oat seed; ortho-Aminobenzoates - analysis; ortho-Aminobenzoates - chemistry; ortho-Aminobenzoates - pharmacology; Other Engineering and Technologies; Plant Extracts - chemistry; Seeds - chemistry; Teknik; Avenanthramides; Avena sativa; Oat seed; Mutagenesis; Antioxidant
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2020.128408

•Mutagenized oat produced lines with high avenanthramides concentration.•Antioxidant activity depends on avenanthramide structures.•The higher concentration of total avenanthramides was 227.5 μg/g oat seed flour. From a mutagenized oat population, produced by ethyl methanesulfonate mutagenesis, hulled grains from 17 lines with elevated avenanthramide (AVN) content were selected and their AVN structures, concentrations and antioxidant potentials were determined by HPLC-MS2 and HPLC equipped with an on-line ABTS+ antioxidant detection system. The data obtained showed qualitative and quantitative differences in the synthesis of AVNs in the different lines, with a total AVN concentration up to 227.5 µg/g oat seed flour in the highest line, compared with 78.2 µg/g seed in the commercial line, SW Belinda. In total, 25 different AVNs were identified with avenanthramide B structures being among the most abundant, and AVN C structures having the highest antioxidant activity. The findings indicate the potential of oat mutagenesis in combination with a high precision biochemical selection method for the generation of stable mutagenized lines with a high concentration of total and/or individual AVNs in the oat seed grain.