Generation of mouse models of lymphoid neoplasm using retroviral gene transduction of in vitro–induced germinal center B and T cells
Evidence is accumulating that hematologic malignancies develop following acquisition of multiple genetic changes. Despite providing many insights into the way by which given genetic changes contribute to the development of disease, the generation of animal models is often laborious. We show a simpli...
Saved in:
Published in | Experimental hematology Vol. 41; no. 8; pp. 731 - 741.e9 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier Inc
01.08.2013
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Evidence is accumulating that hematologic malignancies develop following acquisition of multiple genetic changes. Despite providing many insights into the way by which given genetic changes contribute to the development of disease, the generation of animal models is often laborious. We show a simplified method that allows the retroviral transduction of genes of interest into mouse B or T cells, thus leading to the rapid generation of models of lymphoid neoplasm in mice. Specifically, germinal center B cells induced in vitro from naive mouse B cells and infected with retroviruses for Myc and Bcl2 rapidly developed a neoplasm of immunoglobulin-expressing mature B cells in transplanted mice. Likewise, T cells induced in vitro from immature hematopoietic cells and infected with retroviruses for Myc , Bcl2 , and Ccnd1 rapidly developed CD4+ CD8– and CD4+ CD8+ T cell neoplasm in transplanted mice. These findings support the use of our simplified method as a versatile tool for lymphoma research. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0301-472X 1873-2399 |
DOI: | 10.1016/j.exphem.2013.04.001 |