Fluorescence ratio thermometry in a microfluidic dual-beam laser trap

The dual-beam laser trap is a versatile tool with many possible applications. In order to characterize its thermal properties in a microfluidic trap geometry we have developed a non-intrusive fluorescence ratio technique using the temperature sensitive dye Rhodamine B and the temperature independent...

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Bibliographic Details
Published inOptics express Vol. 15; no. 23; pp. 15493 - 15499
Main Authors Ebert, Susanne, Travis, Kort, Lincoln, Bryan, Guck, Jochen
Format Journal Article
LanguageEnglish
Published United States 12.11.2007
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Summary:The dual-beam laser trap is a versatile tool with many possible applications. In order to characterize its thermal properties in a microfluidic trap geometry we have developed a non-intrusive fluorescence ratio technique using the temperature sensitive dye Rhodamine B and the temperature independent reference dye Rhodamine 110. We measured temperature distribution profiles in the trap with submicron spatial resolution on a confocal laser-scanning microscope. The maximum heating in the center of the trap amounts to (13 +/- 2) degrees C/W for a wavelength of lambda = 1064 nm and scales linearly with the applied power. The measurements correspond well with simulated temperature distributions.
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ISSN:1094-4087
1094-4087
DOI:10.1364/oe.15.015493