First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina

• Published in: 3 Biotech Vol. 9; no. 3; p. 74
• Main Authors: Massot, Francisco, Gkorezis, Panagiotis, McAmmond, Breanne, d’Haen, Jan, Van Hamme, Jonathan, Merini, Luciano J., Vangronsveld, Jaco, Thijs, Sofie
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2019; Springer Nature B.V
• Subjects: Acetic acid; Agriculture; Biodegradation; Bioinformatics; Biomaterials; Biosynthesis; Biotechnology; Calcium metabolism; Calcium phosphates; Cancer Research; Chemistry; Chemistry and Materials Science; Deoxyribonucleic acid; DNA; Genes; Genome Reports; Genomes; Glyphosate; Herbicides; Indoleacetic acid; Nucleotide sequence; Ochrobactrum; Phosphonates; Phosphorus; Phosphorus metabolism; Solubilization; Stem Cells; Argentina; 5-Enolpyruvylshikimate-3-phosphate synthase; Plant growth promotion; Glyphosate; Soil contamination; Ochrobactrum haematophilum
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-019-1606-y

We report here on a high-quality draft genome sequence of Ochrobactrum haematophilum strain P6BS-III (DSM 106071), a Gram negative, non-sporulating bacterium isolated from a pastureland (Buenos Aires province, Argentina) which had been chronically exposed to the herbicide glyphosate. The genome of 5.25 Mb with a DNA G+C content of 56.63% size was estimated to contain 5,291 protein coding genes and 57 RNA genes. Genome analysis revealed the presence of the phn operon, which is involved in the phosphonate degradation pathway, and a class II 5-enolpyruvylshikimate-3-phosphate synthase (EPSP) that confers tolerance to glyphosate. Genes related to plant growth promotion traits are also present, and include genes for phosphorus metabolism, calcium phosphate and phytate solubilization, siderophore production, organic acid biosynthesis and indole acetic acid (IAA) production.