New potent accelerator of neurite outgrowth from Lawsonia inermis flower under non-fasting condition

• Published in: Journal of natural medicines Vol. 70; no. 3; pp. 384 - 390
• Main Authors: Oda, Yoshimi, Nakashima, Souichi, Nakamura, Seikou, Yano, Mamiko, Akiyama, Masanori, Imai, Kayo, Kimura, Tomohito, Nakata, Akiko, Tani, Miyuki, Matsuda, Hisashi
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.07.2016
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Complementary & Alternative Medicine; Flowers - chemistry; Lawsonia Plant - chemistry; Medicinal Chemistry; Neuronal Outgrowth - physiology; Original Paper; PC12 Cells; Pharmacology/Toxicology; Pharmacy; Plant Extracts - chemistry; Plant Sciences; Rats; Vav3; PC12; Henna; Neurite outgrowth; Quercetin; Lawsonia inermis
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-016-0974-4

The methanolic extract of Lawsonia inermis L. (henna) showed accelerative effects on nerve growth factor-induced neurite outgrowth in PC12 cells under non-fasting conditions. To elucidate the active constituents responsible for the neuronal differentiation, we conducted a search of the constituents and examined their accelerative effects on neurite outgrowth in PC12 cells. We isolated a new acetophenone glycoside, inermioside A, which exerted a significant accelerative effect on neurite outgrowth. We also confirmed the activities of nine known compounds, including quercetin and lalioside. In addition, we found that quercetin, one of the active constituents, increased Vav3 mRNA expression.