The effects of vaccination with tissue culture-derived viral vaccines on detection of antibodies to equine arteritis virus by enzyme-linked immunosorbent assay (ELISA)

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of serum antibodies to equine arteritis virus (EAV). Results from this assay produced a good correlation with results from virus neutralisation tests in horses which had not been regularly vaccinated with commercially avail...

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Published inVeterinary microbiology Vol. 20; no. 2; pp. 181 - 189
Main Authors Cook, R.F., Gann, S.J., Mumford, J.A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.06.1989
Elsevier Science
Subjects
Animals
ANTIBODIES
Antibodies, Viral - analysis
ANTICORPS
ANTICUERPOS
ARTERITE
ARTERITIS
Biological and medical sciences
CABALLOS
Centrifugation, Density Gradient
CHEVAL
ELISA
Enzyme-Linked Immunosorbent Assay
False Positive Reactions
Fundamental and applied biological sciences. Psychology
HORSES
Horses - immunology
Microbiology
Neutralization Tests
TEST ELISA
Togaviridae - immunology
VACCIN
Vaccination - veterinary
VACCINES
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
VACUNA
Viral Vaccines - immunology
Virology
VIROSE
VIROSES
VIROSIS
Pestivirus
Immune response
Antibody
Vaccination
Togaviridae
Vaccine
Virus
Characterization
Vertebrata
Mammalia
Antiserum
Horse
Equine arteritis virus
Perissodactyla
Detection
ELISA assay
Ungulata
Humoral immunity
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Summary:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of serum antibodies to equine arteritis virus (EAV). Results from this assay produced a good correlation with results from virus neutralisation tests in horses which had not been regularly vaccinated with commercially available mammalian tissue culture-derived viral vaccines. Vaccination of some horses with tissue culture-derived vaccines induced the formation of antibodies to bovine serum. These antibodies reacted with the bovine protein contaminants in the EAV ELISA antigen, producing false-positive results. Non-viral protein contaminants were found to be closely associated with EAV in that they co-purified with the virus during gradient centrifugation.
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8903876
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ISSN:0378-1135
1873-2542
DOI:10.1016/0378-1135(89)90041-2