Cardiac Type cGMP-Inhibited Phosphodiesterase (PDE3A) Gene Structure: Similarity and Difference to Adipocyte Type PDE3B Gene

• Published in: Biochemical and biophysical research communications Vol. 268; no. 3; pp. 827 - 834
• Main Authors: Kasuya, Junko, Liang, Shu-Jian, Goko, Hideki, Park, Sun-Hee, Kato, Komei, Xu, Zhao-Dong, Hockman, Steve, Manganiello, Vincent C., Fujita-Yamaguchi, Yoko
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.02.2000
• Subjects: 3',5'-Cyclic-AMP Phosphodiesterases - genetics; Adipocytes - enzymology; Base Sequence; Catalytic Domain - genetics; Chromosome Mapping; Chromosomes, Human, Pair 12 - genetics; Cloning, Molecular; Cyclic Nucleotide Phosphodiesterases, Type 3; DNA Primers - genetics; Exons; Female; Humans; In Situ Hybridization, Fluorescence; Introns; Isoenzymes - genetics; Molecular Sequence Data; Myocardium - enzymology; PDE3B gene; Pregnancy; Tissue Distribution; Transcription, Genetic
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2000.2226

Phosphodiesterase type 3 isoforms, PDE3A and 3B, are expressed primarily in cardiovascular and adipose tissues, respectively. We previously reported a shorter transcript of 4.4-kb PDE3A which is predominantly transcribed in human placenta, whereas a full-length 7.6-kb transcript corresponding to the cardiac PDE3A cDNA has not been characterized. Due to unfortunate circumstances created by changes in PDE3 nomenclature, PDE3B gene structure previously reported used PDE3A in its title. Here, we describe PDE3A gene structure, which comprises 16 exons spanning over 130 kb on chromosome 12p12. Two PDE3 isoforms share similar gene organization, but localize to different chromosomes. The most distal transcription initiation site of the PDE3A gene is ∼1071 bases upstream of the ATG site, suggesting that exon 1 consists of 1071 and 960 bp of untranslated and translated sequences, respectively. The proximal 5′-flanking region, which does not contain TATA-like sequences, exhibited weak but significant promoter activity. Results suggest potential involvement of distal promoter/enhancer and translational regulation for expression of the 7.6-kb transcript.