Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simpl...

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Published inJournal of microbiological methods Vol. 105; pp. 98 - 101
Main Authors Hoyos-Mallecot, Y., Riazzo, C., Miranda-Casas, C., Rojo-Martín, M.D., Gutiérrez-Fernández, J., Navarro-Marí, J.M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.10.2014
Subjects
Bacteremia - diagnosis
Bacteremia - microbiology
Bacterial Proteins - analysis
Bacteriological Techniques - methods
beta-Lactamases - analysis
Blood - microbiology
Blood culture
Carbapenemases
Enterobacteriaceae
Enterobacteriaceae - enzymology
Enterobacteriaceae - isolation & purification
Humans
MALDI-TOF
Pseudomonas aeruginosa
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - isolation & purification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
MALDI-TOF
Blood culture
Carbapenemases
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Abstract MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity. •MALDI-TOF detected and identified carbapenemases carrying strains from blood culture.•Predicting the presence of carbapenemases can be useful for a suitable treatment.•Carbapenemase detection and pathogen identification in 4.5h•Excellent values of sensitivity (100%) and specificity (90%) were obtained.
AbstractList MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity.
MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity. •MALDI-TOF detected and identified carbapenemases carrying strains from blood culture.•Predicting the presence of carbapenemases can be useful for a suitable treatment.•Carbapenemase detection and pathogen identification in 4.5h•Excellent values of sensitivity (100%) and specificity (90%) were obtained.
Author Rojo-Martín, M.D.
Hoyos-Mallecot, Y.
Miranda-Casas, C.
Gutiérrez-Fernández, J.
Navarro-Marí, J.M.
Riazzo, C.
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Keywords MALDI-TOF
Blood culture
Carbapenemases
Language English
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Snippet MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase...
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SubjectTerms Bacteremia - diagnosis
Bacteremia - microbiology
Bacterial Proteins - analysis
Bacteriological Techniques - methods
beta-Lactamases - analysis
Blood - microbiology
Blood culture
Carbapenemases
Enterobacteriaceae
Enterobacteriaceae - enzymology
Enterobacteriaceae - isolation & purification
Humans
MALDI-TOF
Pseudomonas aeruginosa
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - isolation & purification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
Title Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS
URI https://dx.doi.org/10.1016/j.mimet.2014.07.016
https://www.ncbi.nlm.nih.gov/pubmed/25063679
https://search.proquest.com/docview/1561131178
https://search.proquest.com/docview/1639994870
Volume 105
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