Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

• Published in: Journal of microbiological methods Vol. 105; pp. 98 - 101
• Main Authors: Hoyos-Mallecot, Y., Riazzo, C., Miranda-Casas, C., Rojo-Martín, M.D., Gutiérrez-Fernández, J., Navarro-Marí, J.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2014
• Subjects: Bacteremia - diagnosis; Bacteremia - microbiology; Bacterial Proteins - analysis; Bacteriological Techniques - methods; beta-Lactamases - analysis; Blood - microbiology; Blood culture; Carbapenemases; Enterobacteriaceae; Enterobacteriaceae - enzymology; Enterobacteriaceae - isolation & purification; Humans; MALDI-TOF; Pseudomonas aeruginosa; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Time Factors; MALDI-TOF; Blood culture; Carbapenemases
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2014.07.016

MALDI-TOF MS has been evaluated to detect carbapenemases activity and pathogen identification directly from positive blood cultures. 21 non-carbapenemase producers and 19 carbapenemase producers Enterobacteriaceae and Pseudomonas aeruginosa strains were included in the study. This technique is simple and detects carbapenemases in 4.5h with high sensitivity and specificity. •MALDI-TOF detected and identified carbapenemases carrying strains from blood culture.•Predicting the presence of carbapenemases can be useful for a suitable treatment.•Carbapenemase detection and pathogen identification in 4.5h•Excellent values of sensitivity (100%) and specificity (90%) were obtained.